The Detection of Apoptosis in Protoplasts from B37 Homocaryotic Multicytoplasm Genotype Maize Induced by HMC-Toxin

doi:10.7668/hbnxb.2011.06.026

Abstract

Abstract: Apoptosis in protoplasts and leaves from Homokaryon maize B37 treated by HMC toxin were studied． The apoptotic features such as apoptotic bodies，chromatin margination or ring chromatin were found after the treatment stained by Hoechst33258． When treated by the same concentration of HMC toxin， the apoptosis rate of B37-C maize protoplasts were significantly higher than B37-N． In addition， the apoptosis rate was increased when the treatment time and concentration increasing． For the leaves of maize B37-C， the obvious DNA ladder was appeared with treatment concentration: 200 μg /mL; treatment time: 6 h． For the leaves of maize B37-N， the obvious DNA ladder was appeared with treatment concentration: 200 μg /mL; treatment time: 9 h which was longer than leaves of maize B37-C． Apoptosis in protoplasts from Homokaryon maize B37 were same to the leaves． B37-C was more sensitive than B37-N．

Key words: Helminthosporium maydisrace C(HMC) toxin, Apoptosis, Hoechst33258, DNA ladder

CLC Number:

S513． 01

MA Chun-hong, LI Xiu-li, DONG Wen-qi, JI Zeng-fa, LI Xiao-yu, DAI Zhi-gang, WANG Li-an, JIA Yin-suo. The Detection of Apoptosis in Protoplasts from B37 Homocaryotic Multicytoplasm Genotype Maize Induced by HMC-Toxin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 136-140. doi: 10.7668/hbnxb.2011.06.026.

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http://www.hbnxb.net/EN/Y2011/V26/I6/136

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