Response of Ultrastructure and Ca2+Distribution to Low Temperature in Leaves of Cabbage Seedlings

doi:10.7668/hbnxb.2011.06.025

Abstract

Abstract: The objective of the study is to investigate the effects of low temperature stress on the leaf ultrastructure and Ca²⁺distribution of various cabbage varieties，and to find the different response mechanisms between them． The seedlings were treated separately under adverse temperature conditions in growth chambers，and the effects on cell ultrastructure and Ca²⁺distribution of cabbage leaves under low temperature stress were studied，and various cabbage varieties was compared． Under 5℃ cold stress，most leaf cell organelles of cabbage were not injured and orderly arranged． This sugGested that both cabbage varieties adapted to 5℃ cold stress． Under-3℃ cold stress， the ultrastructure of leaf cell in cold-resistant lines had little destructiveness，whereas， the grana lamella of leaf cell in cold-sensitive lines were disorganized and mitochondrion's cristae could not be found． Under optimum temperature (25 /18℃) conditions，calcium antimonite deposits，which indicates calcium distribution，were mainly localized in vacuoles and intercellular space， and few was found in cell wall or nucleus． At controlled temperature of-3℃ for 24 h， an elevation of Ca²⁺level in the cytosol and nucleus was observed in two varieties，wheas the cold-sensitive lines had few than cold-resistant lines． By chilling up to 48 and 72 h， the intracellular Ca²⁺concentration had been restored to a similar low level as the status as treatment before． On the contrary， the Ca²⁺concentration of the cold-sensitive variety increased continuously to the maximal level within the whole range of temperature changes． This study indicated that there were distinctive differences between the cabbage species in cell ultrastructure and Ca²⁺distribution， It could be seen that cabbage cold-hardiness was related to the changes in cell ultrastructure and Ca²⁺distribution．

Key words: Cold temperature, Cabbage, Cell ultrastructure, Ca²⁺distribution

CLC Number:

S635． 01

ZENG Ai-song, YAN Ji-yong, SONG Li-xiao, GAO Bing, FENG Cui. Response of Ultrastructure and Ca²⁺Distribution to Low Temperature in Leaves of Cabbage Seedlings[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 129-135. doi: 10.7668/hbnxb.2011.06.025.

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