Abstract: Porcine bocavirus(PBoV) was a recently discovered parvovirus from pigs.One pair of primers was designed according to the published sequences of genome of the porcine bocaviruses(GenBank accession number GU902967-GU902971).The NP1 gene was amplified by PCR and cloned into the prokaryotic expression vector pET32a(+) to construct a recombinant pET32a-NP1.After sequencing,the recombinant was transformed into Escherichia coli BL21(DE3) competent cells.The transformed bacteria were induced by IPTG to produce a recombinant protein of 46 kDa of molecular weight.The result showed that recombinant protein was higher expression and purity by the Ni-NTA resin,and in soluble form in the bacteria.The recombinant protein could react with the antibody of His in a Western blot test,indicating that the expressed protein possessed strong reactinogenicity.

Key words: Porcine bocavirus, NP1 gene, Cloning, Prokaryotic expression

CLC Number: 

• Q785

• S855.3