Preliminary Study on the Effects of BmDHS Gene Silencing by RNAi on Growth and Development of the Silkworm, Bombyx mori

doi:10.7668/hbnxb.2011.02.003

Abstract

Abstract: By using mulberry antifreeze protein gene'dsRNA and autoclaved distilled water as control based on microinjection RNA interference technology, we have prelin inarily got BmDHS gene silence phenotype by injecting 4th larval instar. Compared with control group, in the silent silk worm, the average level of BmDHS mRNA was reduce almost 25%; the average level of BmelF5A mRNA was reduced about 4.8%;the average larval weight increased by 42.9%.10 days after injection; the average cocoons weght increased by 35.4% at the fifth day after mounting. It prelim inarily proofed that BmDHS gene plays an inportant role in silk worm's growth and development.

Key words: Bambyx mori, BmDHS, Growth and development, RNAi

CLC Number:

S881.2
S186

WANG Geng-xian, SIMA Yang-hu, ZHANG Sheng-xiang, XU Shi-qing. Preliminary Study on the Effects of BmDHS Gene Silencing by RNAi on Growth and Development of the Silkworm, Bombyx mori[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 13-16. doi: 10.7668/hbnxb.2011.02.003.

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