Isolation, Identification and 16S rDNA Sequences Analysis of a Rhizobium Strain Resistant to Copper

doi:10.7668/hbnxb.2011.02.002

Abstract

Abstract: In recent years, with the development of mining smeltilg and processing of copper industrg soi1 copper pollution is becoming increasingly serious. Thus it is imperative to restore the soil copper contan ination.In this study,in order to develop collaboratiae restoratim activity of microorganisns and plants in the copper-contam inated soils of heavy metals tailing areas an excellent Rhizobiwn strain resistance to copper was screened from mining tailings Fengxian county, Shaanxi province. The strain named CCNW SX0020, resisted to 30 mmo1/L Cu²⁺ on YMA agar plate and to 14 mmol/L Cu²⁺ in TY liquid medium, so it is an important strain using to study the resistant copper mechanisms of microbe.It was prelin inarily identified as a strain of Sinorhizobiun meliloti, based on its physilogical and biochemical characteristics analysis including G+C mol% detemination,DNA homology hybridization and 16S rDNA sequence similarity comparison. The sinilarity betveen CCNW SX0020 and S. meliloti IMG6133(GenBank accession number X67222) was 99.%. Because of Rhizobia with the unique biology status that is being ab1e to form symbiont with legume, which can provide a good genetic base for the further construction of a biology system being tolerant to heavy metals, and as a resu1t, having potential ecological and social benefits.

Key words: Heavymetals, Copper, Rhizobia, 16S rDNA

CLC Number:

Q936

FAN Lian-mei, LI Zhe-fei, WEI Ge-hong. Isolation, Identification and 16S rDNA Sequences Analysis of a Rhizobium Strain Resistant to Copper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 7-12. doi: 10.7668/hbnxb.2011.02.002.

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