Construction of Rice TINA Insertion Population and the Acquisitionand Analysis of Flanking Sequence

doi:10.7668/hbnxb.2011.01.007

Abstract

Abstract: Abstract:In this study the method of Agrobacterium-mediated transformation was used to transform GUS genecarried by plasmid 1301 into Nipponbare and 5200 rice TINA insertion population had been established. Studiesshowed that transgenic plants had been proved to be transformed by methods of PCR and Southern Blot and 437 flan-king sequence had been isolated by improving method of TAILCR. By sequence homology analysis showed that therewere 147 sequences containing only the vector sequence，and 214 sequences containing TINA right border with theneighboring ricesequences and public databases in the rice genomesequence，threegenome sequence. By comparison of the bordermutants TINA insertion sites had been locatedin a particular rice chromosome. These results willprovide important information for functional genomics research.

Key words: TINA, Insertional mutaenesis, Transenic ICeTAILCR

CLC Number:

S511.035.3

WEI Jin-zhao, CHEN Qiu-ling, PEI Zhong-you, SUN Shou-jun. Construction of Rice TINA Insertion Population and the Acquisitionand Analysis of Flanking Sequence[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 31-36. doi: 10.7668/hbnxb.2011.01.007.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.01.007

http://www.hbnxb.net/EN/Y2011/V26/I1/31

[1]	WANG Yuanhong, BAI Caixia, YANG Kankan, ZHANG Yuchen, YU Zhaorong, WANG Yong, CAI Haiying. Mechanism of Green Tea Powder Improving Intestinal Barrier of Immune Stressed Mice [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 136-142.
[2]	ZHANG Jiao, WANG Zhenhua, PAN Kangcheng, NI Xueqin, LI Jiajun, LI Jianzhen, GU Xiaoxiao. *Effects of Bacillus cereus* PAS38 on Growth Performance and Immune Function of Broilers** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 340-345.
[3]	TENG Yuxi, ZHANG Hongyan, XI Wendi, LIANG Shiyu, FANG Yulin, WANG Hua, JIN Guojie. Screening of Bio-control Bacteria Against Grape Mycosis Based on the Method with Substrate Stress [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 253-258.
[4]	GUO Yue, JIANG Pingping, PAN Leilei, ZHOU Wenjie, XU Lei, ZHANG Ruqin, SUI Jiongming, GUO Baotai, WANG Jingshan, QIAO Lixian. Cloning and Disease Resistance Verification of Chitinase Gene in Peanut [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 42-48.
[5]	GONG Shasha, MENG Qingling, QIAO Jun, ZHONG Wenqiang, HUANG Yunfu, ZHANG Guowu, CHEN Ying, CAI Xuepeng. *Cloning and Expression of Chitinase Gene AO-190* of Arthrobotrys oligospora XJ-A1 and Analysis of the Chitinase Activity of Recombinant Protein** [J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 67-74.
[6]	LI Pengpeng, WU Yundong, YE Jia, LI Danhua, QIAO Lijuan, ZHANG Yuxing, DONG Jingao. *Cloning and Expression Analysis of PbChi Ⅱ* from Pyrus bretschneideri Yali** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 45-51.
[7]	ZHAO Chunmei, FAN Xi, XUE Rengao. *Molecular Cloning and Function Analysis of a GmCHI1* Promoter** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 32-36.
[8]	WAN Ye, GAO Jing, WANG Feng-ru, DONG Jin-gao. Effects of BR Treatment on the Chitinase Activity of the Intercellular Fluid in Maize [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 360-363.
[9]	ZHU Xin-chan, WANG Qian-wen, YU Qun. Isolating and Bio-characterization of Chitinase from Momordica charantia [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 182-187.
[10]	WANG Kai, ZHAO Yang, ZHU Xian-ting, CAI Xiu-hua, WU Xia. *Cloning and Analysis of Chitinase Gene IFCHI1* from Egg-parasitic Fungus Isaria farinosa** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 78-86.
[11]	SUN Ying-feng, WANG Li-li, LU Chao, LI Xiu-li, ZHANG Li, YANG Chun-lei, FENG Jing, REN Wei-ke, TIAN Xiang-xue. Effect of Sulfated Yeast β-glucan on Hemagglutination and mRNA Transcription of H1N1 Swine Influenza Virus in vitro [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 15-18.
[12]	ZHAO Yang, CHEN De-xin, WANG Feng-long, HUANG Hua-gang, WU Xia. Identification of Fungus Lecanicillium attenuatum and Cloning of Its Chitinase Gene LACHI1 [J]. journal1, 2014, 29(4): 63-70.
[13]	YAN Rui-xiang, ZHANG Na, GUAN Wen-qiang, YU Jin-ze. Antifungal Activity of Chitinase on the Several Pathogen from Fruit & Vegetable in vitro [J]. journal1, 2014, 29(4): 155-159.
[14]	SUN You-he, ZHOU Kai-yan, XIONG Zhi. *Screening and Identification of Cellulase-producing Strains from Intestinal Canal of Dendrolimu houi* and Research on Cellulase Characterization** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 254-258.
[15]	YAN Rui-xiang, LI Ning. Effect of the Recombinant Chitinase on the Disease Resistance in Table Grape during Postharvest Storage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 158-162.

Please choose a citation manager

Content to export