Cloning and Sequence Analysis of ACC Oxidase Gene 1 cDNA from Melon Cultivar Hetao(Cucumis melo L.cv Hetao)

doi:10.7668/hbnxb.2010.04.013

Abstract

Abstract: A pair of specific primer was designed based on melon ACC oxidase gene Cm-ACO1 in Genbank. The full-length cDNA of ACO1 was cloned by RT-PCR from ripening fruit of melon (Cucumis melo L.cv. Hetao) . The length of cDNA was 1 035 bp. Sequence analysis indicated that the cDNA sequence was consistent with that of reported melon ACO1 cDNA. This work may contribute to study the expression pattern and function of ACO1 in melon.

Key words: Melon, ACC oxidase gene1, cDNA

CLC Number:

Q78
S652.1

HAO Jin-feng, GAO Feng, BO Yan-tai, LI Feng, Hasi Agula. Cloning and Sequence Analysis of ACC Oxidase Gene 1 cDNA from Melon Cultivar Hetao(Cucumis melo L.cv Hetao)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 61-63. doi: 10.7668/hbnxb.2010.04.013.

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