Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs

doi:10.7668/hbnxb.2010.01.012

Abstract

Abstract: A full length cDNA library of Hoplobatrachus rugulosus skin tissue was constructed with the SMART technique.A special primer was designed from a highly conserved domain of the signal peptide of antimicrobial peptide encoding cDNAs from ranid frogs.Two different cDNA sequences encoding precursor proteins named tigerinin-HR1 and tigerinin-HR2 were screened from the library by PCR.tigerinin-HR1 and tigerinin-HR2 were the first found encoding cDNA sequences of antimicrobial peptide from H.rugulosus.

Key words: Hoplobatrachus rugulosus, cDNA library, Antimicrobial peptides, tigerinin

CLC Number:

Q785

WANG Hui1,2,ZHANG Xiu-qing1,HU Yu-hong3,SUN Jun-she1. Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 61-67. doi: 10.7668/hbnxb.2010.01.012.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.01.012

http://www.hbnxb.net/EN/Y2010/V25/I1/61

References

[1] 罗琛,刘楚吾,姚占州.虎纹蛙人工养殖的研究初报[J].湖南师范大学:自然科学学报,1992,15(2):175-178.
[2] Ganz T.Defensins:antimicrobial peptides of innate immunity[J].Nat Rev Immunol,2003,3(9):710-720.
[3] Skerlavaj B,Benincasa M,Risso A,et al.SMAP-29:A potent antibacterial and antifungal peptide from sheep leucocytes[J].FEBS Lett,1999,463(1-2):58-62.
[4] Wang H X,Ng T B.Isolation and characterization of an antifungal peptide with antiproliferative activity from seeds of Phaseolus vulgaris cv.'Spotted Bean'[J].Appl Microbiol Biotechnol,2007,74(1):125-130.
[5] Wu S,Jia S,Sun D,et al.Purification and characterization of two novel antimicrobial peptides Subpeptin JM4-A and subpeptin JM4-B produced by Bacillus subtilis JM4[J].Curr Microbiol,2005,51(5):292-296.
[6] Simmaco M,Mignogna G,Canofeni S,et al.Temporins,antimicrobial peptides from the European red frog Rana temporaria[J].Eur J Biochem,1996,242(3):788-792.
[7] Morikawa N,Hagiwara K,Nakajima T.Brevinin-1 and-2,unique antimicrobial peptides from the skin of the frog,Rana brevipoda porsa[J].Biochem Biophys Res Commun,1992,189(1):184-190.
[8] Simmaco M,Mignogna G,Barra D,et al.Antimicrobial peptides from skin secretions of Rana esculenta.Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides[J].J Biol Chem,1994,269(16):11956-11961.
[9] Isaacson T,Soto A,Iwamuro S,et al.Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica[J].Peptides,2002,23(3):419-425.
[10] Rao X C,Li S,Hu J C,et al.A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli[J].Protein Expr Purif,2004,36(1):11-18.
[11] Xu Z,Peng L,Zhong Z,et al.High level expression of a soluble functional antimicrobial peptide,human beta-defensin 2,in Escherichia coli[J].Biotechnol Prog,2006,22(2):382-386.
[12] Sai K P,Jagannadham M V,Vairamani M,et al.Tigerinins:novel antimicrobial peptides from the Indian frog Rana tigerina[J].J Biol Chem,2001,276(4):2701-2707.
[13] Song Y,Lu Y,Wang L,et al.Purification,characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora[J].Peptides,2009,30(7):1228-1232.
[14] Chen T,Li L,Zhou M,et al.Amphibian skin peptides and their corresponding cDNAs from single lyophilized secretion samples:identification of novel brevinins from three species of Chinese frogs[J].Peptides,2006,27(1):42-48.
[15] Suzuki H,Iwamuro S,Ohnuma A,et al.Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii[J].Peptides,2007,28(3):505-514.
[16] Mangoni M L.Temporins,anti-infective peptides with expanding properties[J].Cell Mol Life Sci,2006,63(2006):1060-1069.
[17] Park Y,Lee D G,Jang S H,et al.A Leu-Lys-rich antimicrobial peptide:activity and mechanism[J].Biochim Biophys Acta,2003,1645(2):172-182.
[18] Huang L,Ching C B,Jiang R,et al.Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression[J].Protein Expr Purif,2008,61(2):168-174.
[19] Zhou M,Chen T,Walker B,et al.Pelophylaxins:novel antimicrobial peptide homologs from the skin secretion of the Fukien gold-striped pond frog,Pelophylax plancyi fukienensis:identification by "shotgun" cDNA cloning and sequence analysis[J].Peptides,2006,27(1):36-41.
[20] Chen T,Zhou M,Rao P,et al.The Chinese bamboo leaf odorous frog(Rana(Odorrana)versabilis)and North American Rana frogs share the same families of skin antimicrobial peptides[J].Peptides,2006,27(7):1738-1744.
[21] Zhou M,Chen T,Walker B,et al.Lividins:novel antimicrobial peptide homologs from the skin secretion of the Chinese Large Odorous frog,Rana(Odorrana) livida.Identification by "shotgun" cDNA cloning and sequence analysis[J].Peptides,2006,27(9):2118-2123.
[22] 王辉,张秀清,胡玉红,等.阔褶水蛙皮肤组织cDNA文库的构建及抗菌肽brevinin 2-LTs的cDNA克隆和序列分析[J].华北农学报,2009,24(增刊):9-14.
[23] Frost D R,Grant T,Faivovich J,et al.The amphibian tree of life[J].Bull Am Mus Nat His,2006,297:1-370.
[24] Conlon J M,Kolodziejek J,Nowotny N,et al.Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog,Rana dybowskii[J].Toxicon,2007,50(6):746-756.
[25] Conlon J M,Kolodziejek J,Nowotny N.Antimicrobial peptides from ranid frogs:taxonomic and phylogenetic markers and a potential source of new therapeutic agents[J].Biochimica Biophysica Acta,2004,1696(1):1-14.

Please choose a citation manager

Content to export