Abstract: We choose BMP15 gene which affect Invedal and Hanna awaits prolificacy trait as candidate gene of prolificacy function,designed to explore the relationship between the candidate gene and lamb reproductive traits of Mongolian sheep,provide a scientific basis for carrying out the research of Mongolia sheep high fecundity genetics.Taking the single-lamb Mongolian sheep group(18),multiple births type of Small Tail Han sheep(30) as contrast groups of double-lambs Mongolian sheep group(50),using the molecular marker technique polymerase chain reaction-restriction fragment length polymorphis(Restriction Fragment Polymorphisms,RFLPs)analysis genetic polymer-phism of the FecXI site in the BMP15 gene;using the single nucleotide polymorphism PCR-SSCP(Single strand confomation polymorphim) technology for the B1 site in BMP15 gene polymorphism analysis.The results showed:FecXI site mutation was absent in the Mongolian sheep double-lambs group,the single-lamb Mongolian sheep group and the Small Tail Han group.That is,FecX^I site in the BMP15 gene isn′t the major gene for high fecundity that affect Mongolia sheep and Small Tail Han sheep.The B1 mutation 28-30 bp(CTT deletion) existe in the three groups and found three genotypes.The genotype frequencies were 0.020(++),0.940(B+)and 0.040(BB) in the Mongolian sheep double-lambs group;Just found tow genotypes in Mongolian sheep single-lamb group,the genotype frequencies were 0.722(++),0.278(B+);0.300(++),0.667(B+)and 0.033(BB)in Small Tail Han Sheep.The average litter size in Mongolian sheep ewe group genotype B+ was higher 0.83 than ++ genotype ewes,the difference is verry significant(P<0.01),inferred the B1 mutation maybe is the major gene to control the high fecundity in Mongolian sheep double-lambs group;Small Tail Han sheep genotype group ewes average litter size was not significant difference(P>0.05).By the x² test showed that the Mongolian sheep double-lambs group significant deviations from Hardy-Weinberg equilibrium(P<0.01);the locus of Mongolian sheep single-lamb group and Small Tail Han Sheep in Hardy-Weinberg equilibrium(P>0.05).

Key words: Sheep, Fecundity, BMP15 gene

CLC Number: 

• S826