Screening and Identification of B Cell Epitopes of 3D Polymerase of FMDV

doi:10.7668/hbnxb.2009.03.015

Abstract

Abstract: Foot2and2mouth disease virus strain AF72 RNAs were used as templates for RT2PCR to amplify the 3D gene. The purified PCR products were cloned into pGEm²T easy vectors and transformed into E. coli JM109. The positive recombinant plasmids identified by electrophoresis,PCR,and EcoR Ⅰ cleavage were sequenced. The nucleotide and amino acid sequence were obtained by comparing with the full2length sequence of the other reference strains. Potential B cell epi2 topes of 3D were predicted and epitope peptide segments were synthesized. They were identified by indirect ELISA. The re2 sult showed that 3D2 and 3D4 were predominant B cell epitope of 3D,it provided a basisfor further studyon the multi2epi2 tope vaccine of FMD.

Key words: FMDV, 3D, Indirect ELISA, B2cell epitope

CLC Number:

ZHANG Yu, WANG Yong lu, ZHANG Yong guang, PAN Li, FANG Yu zhen, LIU Li kuan, JIANG Shou tian, LU Jian liang, ZHANG Zhong wang, ZHANG Shugang, LI Zheng feng, DU Jin xin. Screening and Identification of B Cell Epitopes of 3D Polymerase of FMDV[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 69-73. doi: 10.7668/hbnxb.2009.03.015.

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http://www.hbnxb.net/EN/Y2009/V24/I3/69

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