Study of Reference Cellular Strain of AF/72 of FMD Type A Virus

doi:10.7668/hbnxb.2009.03.016

Abstract

Abstract: Get the foot2and2mouth virus AF/ 72/ MF₆/BF₁₂by rat rejuvenation and BHK 221 cells adaption using FMD virus AF/ 72/ MF3the use of Karber method to measure AF/ 72/ MF₆/BF₁₂TCID50. its value was 8. 0;the main antigen VP₁ gene sequence was got by RT2PCR with 6 type A strains of FMDV in GenBank,homology was higher than 85 %.By the sterile examination,other exogenous virus test,its purityof AF/ 72/ MF₆/BF₁₂conform to Biological Products Standards for Animal Use;Indirect Sandwich ELISA OD Values greater than 0. 2,This virus was only neutralized by the reference serum of FMD type A in line with the specific;146S antigen content was measured by ultraviolet spectrophotometer,The average value was much higher than 22 ng/ mL of international standards. AF/ 72/ MF₆/BF₁₂was identified as reference cellular strain of AF/ 72 of FMD type A virus integrating many parameters.

Key words: FMD type A virus, FMD, Strain

CLC Number:

DU Jin xin,, WANG Yong lu, ZHANG Yong guang, FANG Yu zhen, JIANG Shou tian, LU Jian liang, PAN Li, LIU Li kuan. Study of Reference Cellular Strain of AF/72 of FMD Type A Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 74-77. doi: 10.7668/hbnxb.2009.03.016.

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