Clonging of PRO Gene from Aromatic Rice and Constructing of Its Expression Vector

doi:10.7668/hbnxb.2009.03.007

Abstract

Abstract: By RT2PCR method the cDNA of PRO gene was obtained. Analysis of DNA sequence showed that the largest open reading frame sequence of PRO cDNA was 1 428 bp,which could encode 476. amino acids. The sequence with the NCBI web site has been published by the PRO gene 100 % similarity. In order to further verify the sequence by which we cloned the gene required for the successful construction of a over expression PRO gene vector and interference expression PRO gene vector,to facilitate the import of rice carried out to identify the function of genes.

Key words: Aromatic rice, Proline oxidase, Gene, Cloning, Expression vector

CLC Number:

Q785

TIAN Hua, XU Chun xia, DUAN Mei yang, LI Guo xi, TANG Xiang ru. Clonging of PRO Gene from Aromatic Rice and Constructing of Its Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 32-36. doi: 10.7668/hbnxb.2009.03.007.

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