Molecular Detection of Wolbachia in Small Brown Planthopper( Laodelphax striatellus）

doi:10.7668/hbnxb.2009.01.039

Abstract

Abstract: Wolbachia bacteria is an obligate intracellular bacterium found in a wide range of invertebrate hosts andcause alterations in host reproductive phenotypes,including cytoplasmic incompatibility,feminization,parthenogenesis,male killing,fitness alterations,and other phenomena. Small brown planthoppers (Laodelphax striatellus) was therst irtrportant vector of some plant virus transmission and the populations were trended to increase in recent years which causedserious damage to rice and maize plant. Thus the infection of Wolbachia in small brown planthoppers from different re-gions was detected by polymerase chain reaction(PCR) using the universal primer 81F and 691Rs of the surface proteingene of Wolbachia(wsp gene,encoding the outer membrane protein of the symbiotic bacteria) in this paper. The resultsdemonstrated that planthoppers from Quyang county and Rongcheng county were infected with Wolbachia and the infectionrate was 85.6%and 88. 4%respectively. The PCR products were sequenced and analyzed. It was showed that a region of601-603of Wolbachia wsp gene were amplified from the total DNA of small brown planthopper and there was 100%nucleotide identity with other strains derived from Sogatella fureifera and Laodelphax striatellus. It indicated that the wol-bachia infected in small brown planthopper from Hebei province had close relatives with the strain wFur (derived from So-gatellafureifera) and wStri (derived from Laodelphax striatellus).They all belong to the Con group of B supergroup.Therefore,it was possible that wolbachia infected Laodelphax striatellus derived from the same group.

Key words: Laodelphax striatellus, Wolbachia, Detection

CLC Number:

S435.112.3

DI Dian-ping, MIAO Hong-qin, LU Yin-gui, TIAN Lan-zhi. Molecular Detection of Wolbachia in Small Brown Planthopper( Laodelphax striatellus）[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 178-180. doi: 10.7668/hbnxb.2009.01.039.

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