Abstract: The aim of the research was to establish SRAP??PCR amplification system which was suitable for Peach (Prunus persica) genome DNA so as to lay foundation for the construction of gene map and molecular marker in Peach ( Prunus persica). Peach ( Prunus persica) genome DNA as template, the major components of SRAP, such as concentrations of dNTPs,Mg²⁺, Taq DNA polymerase, primers and template,were optimized in this study by orthogonal design in five factors four levels respectively. The results showed that the optimum SRAP reaction system includes dNTPs 0.12mmol/ L,Mg²⁺ 4 mmol/L, Taq DNA polymerase 2 U, primer 0. 3mmol/ L and DNA template 50 ng in the 25L system.The most suitable protocol was init ially denaturing at 94 for 5 min, then pre??amplifying at 94.1 min, 35.l min and72.1 min for five cycles, finally amplifying for 35 cycles when the annealing temperature was adjusted to 50 ??.

Key words: Peach ( Prunus persica) , SRAP, Optimization, Orthogonal design

CLC Number: 

• S662.1