Abstract:
To construct the optimum SRAP reaction system of Paeonia lactif lora Pall., orthogonal experimental design L25(56) was used.And the comprehensive effect of five levels of five factors ( Taq polymerase,Mg2+, dNTP, primer and DNA template) was assessed.According to the amplificat ion results, treatment 18 was used for the subsequent detection for stability with other 5 cultivars. The final results showed that by orthogonal design, an optimal and stable SRAPPCR system for herbaceous peonies was quickly obtained: 2.5mmol/ L Mg2+, 0.25 mmol/L dNTP, 0.3 mol/L primer,1 U Taq polymerase, 120 ng DNA template within total 25 ??L reaction solution.
Key words:
Paeonia lactif lora Pall. ,
SRAP??PCR,
Orthogonal design
CLC Number:
SUN Xian-zhi, WANG Xiao-han, MA Yan, GUO Xian-feng, YU Xiao-yan. Establishment of the SRAP Reaction System of Paeonia lactiflora Pall.by Orthogonal Design[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 198-200. doi: 10.7668/hbnxb.2008.S2.045.