Optimization and Establishment of SSR Analysis System in Roses(Rosa spp.)

doi:10.7668/hbnxb.2009.03.039

Abstract

Abstract: Using orthogonal design, five factors including concentration of template、 Mg²⁺、 dNTP, primers and Taq DNA polymerase were optimized to establish SSR analysis system in roses.The results showed that 30- 40 ng DNA tem - plate, 1- 1. 5 mmol/L Mg²⁺, 0. 2mmol/ L dNTP, 1 L mol/ L each primer and 1. 5U Taq DNA polymerase in 20 L L reac - tion system were the optimal option.Through the optimized reaction system, SSR fragments of 12 rose cultivars were ob - tained and detected. Polymorphism between different cultivars was abundantly detected by 3% agarose gels,which showed that the system established in this study was suitable and stable.

Key words: Rose, SSR, Orthogonal design, System optimization

CLC Number:

S685.12

BAI Jin rong, PAN Hui tang, ZHANG Qi xiang. Optimization and Establishment of SSR Analysis System in Roses(Rosa spp.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 184-188. doi: 10.7668/hbnxb.2009.03.039.

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