Study the Effect of Pretreatment and Exogenous Hormones on Embryoids Induced from Unpollinated Ovaries of Cucumber

doi:10.7668/hbnxb.2008.S1.013

Abstract

Abstract: The pretreatment, NAA, 2, 4D, BA and KT were investigated in the culture of unpollinated ovaries of cucumber. The result showed that the 36℃ pretreatment could induced high frequency embryoids than the 4℃ pretreatment. The best culture condition of unpollinated ovaries of cucumber were 36℃ 3 d+ 05mg/ L NAA₊ 2.0 mg/ L KT₊ 1.5 mg/ L BA. 2, 4 D, NAA and BA were good for the embryoid inducement.

Key words: Cucumber, Ovary, Pretreatment, Exogenous hormone

CLC Number:

S642.2

WANG Ye, GU Xing-fang, ZHANG Sheng-ping. Study the Effect of Pretreatment and Exogenous Hormones on Embryoids Induced from Unpollinated Ovaries of Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S1): 50-53. doi: 10.7668/hbnxb.2008.S1.013.

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References

[1] 北京农学院园艺系.园艺植物栽培学[M].北京:1998:472.
[2] Gémesné J A,Venczel G,Balogh P.Haploid plant induction in zucchini(Cucurbita pepo L.convar.Iromontiina DUCH) and in cucumber(Cucumis sativus L.) lines through in vitro gynogenesis[J].Acta Hort,1997,447:623-624.
[3] Gémesné J A,Balogh P,Ferenczy A,et al.Effect of optimum stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber(Cucumis sativus L.)[J].Plant Cell Reports,2002,21(2):105-111.
[4] 杜胜利.利用生物技术创造黄瓜育种新材料方法研究[J].天津科技,2001(2):627.
[5] 周维燕.植物细胞工程原理与技术,人工诱发单倍体及其应用[M].北京:中国农业大学出版社,2001:110-115.
[6] 陈学军,邢国明,陈竹君.西葫芦未授粉胚珠离体培养和植株再生[J].浙江农业科学,2000,12(3):165-167.
[7] 雷春,陈劲枫.葫芦科蔬菜作物单倍体材料创制的研究进展[J].中国蔬菜,2006(1):34.
[8] Caglsr G,Abak K.Efficiency of haploid production in cucumber[J].Cucurbit Genet Coop Rpt,1996,19:36-37.
[9] Chambonnet D,Dumas de Vaulx R.Obtention of embryos and plants from in vitro culture of unfertilizered ovules of Cucurbita pepo[J].Cucurbit Genet Coop Rpt,1985(8):66.
[10] Dumas de Vaulx R,Chambonnet D.Obtention of embryos and plants from in vitro culture of unfertilizered ovules of Cucurbita pepo[J].Genetic Manipulation in Plant Breed,1986.295-297.
[11] Metwally E I,Moustafa S A,E1-Sawy B I,et al.Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo[J].Plant Cell,Tissue and Organ Culture,1998,52:117-121.
[12] Nikolova V,Alexandrova M.Gynogenesis in a diploids lines of cucumber(Cucumis sativus L.)[J].Cucurbit Genet Coop Rpt,2001,24:20-2.
[13] Przyborowski J,Niemirowicz Szczytt K.Main factors affecting cucumber(Cucumis sativus L.) haploid embryo development and haploid plant characteristics[J].Plant Breed,1994,112:70-75.
[14] Przyborowski J.Haploidy in cucumber(Cucumis sativus L.)[M] ∥Jain A M,Sopory S K,Veilleux R E.In vitro haploid production in higher plants.The Nertherlands:Kluwer Academic Publishers,1996:91-98.

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