Induction of Insect-resistant Gene into Cucumber by Pollen Tube Pathway and Selection of Kanamycin Resistance

doi:10.7668/hbnxb.2008.06.012

Abstract

Abstract: Effects of Kanamycin of different concentration on cucumber seed germination and seedling growth were studied. Screening method for kanamycin resistant seedling was established. Seeds were sowed first in 1/ 2MS medium containing Km 200 mg/ L,seedling growth could be effectively inhibited. After 10d,root was cut and then transplanted the seedling into Km free vermiculite could enhance seedling declining and dying,and therefore enhanced screening of resistant plants. T₀ seeds from pollen tube pathway transformation were screened by this method,1. 6 % of seedlings were tested as resistant,among them 8.9 % were tested as PCR negative. It primarily certified that EQKAM gene was combined into Cucumber gene group.

Key words: Cucumber (Cucumis sativus L.), Pollen tube pathway, Kanamycin

CLC Number:

Sb42.203.6

WEI Ai-min, ZHANG Wen-zhu, DU Sheng-li, HAN Yi-ke, ZHANG Gui-hua, LIU Nan. Induction of Insect-resistant Gene into Cucumber by Pollen Tube Pathway and Selection of Kanamycin Resistance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 54-57. doi: 10.7668/hbnxb.2008.06.012.

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http://www.hbnxb.net/EN/Y2008/V23/I6/54

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