Clone and Sequencing of NA Genes of 10 H9N2 AIV Strains with Different Ages

doi:10.7668/hbnxb.2008.06.013

Abstract

Abstract: NA genes of 10 H9N2 AIV strains, which cover all isolated ages and different virulence, were cloned by RT- PCR. The sequence analysis showed the homology of nucleotides of NA genes and their deduced amino acids between the 10 AIV strains was 91. 6% - 99. 0% and 91 9% - 100%, respectively, indicating the stead evolution of these chick-en-source H9N2 AIV strains. Phylogenetic analysis suggested that the NA genes of these H9N2 AIVs belonged to eurasia clade and their distribution displayed a certain regional correlation. The changes of potential glycosylation sites and erythrocyt e adsorption sites of the 10 strains could not explain the difference of their biological characteristics. The result of this research not only enriched the molecular epidemiology of H9N2 subtype AIV, but also provided the scient ific base for further studying the evolution of them.

Key words: H9N2 AIV, NA gene, Clone, Sequence analysis

CLC Number:

S858.31
Q785

YANG Da-guang, WANG Xin-wei, WANG Ze-lin, ZHOU Xin. Clone and Sequencing of NA Genes of 10 H9N2 AIV Strains with Different Ages[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 58-63. doi: 10.7668/hbnxb.2008.06.013.

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