Study on Expression of Corn Gene Fragments Related to Resistance to Setosphaeria Turcica

doi:10.7668/hbnxb.2008.04.020

Abstract

Abstract: The expression of gene fragments in the corn leaves were studied both in incompatible combination(B37 Ht1 inoculated with race 0 of Setosphaeria turcica ) and in compatible combination(B37 Ht1 inoculated with race 1 of Setosphaeria turcica ) by mRNA differential display PCR.The results showed that 17 gene fragments were expressed ex-clusively,26 gene fragments expressed increasingly,and 20 gene fragments expressed decreasingly in incompatible combi-nation compared with either in compatible combination or in control. Homology was not found between gene fragments ex-pressed exclusively in incompatible combination and known R genes.

Key words: Corn, Northern corn leaf blight, mRNA differential display reverse transcription PCR(DDRT 2 PCR), gene fragments related to disease resistance

CLC Number:

S435.13

HAO Min, GU Shou-qin, ZHANG Ying, DONG Jin-gao, HAN Jian-min. Study on Expression of Corn Gene Fragments Related to Resistance to Setosphaeria Turcica[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 85-88. doi: 10.7668/hbnxb.2008.04.020.

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