Cloning and Sequence Analysis of F Gene of Newcastle Disease Virus Isolated from Hebei Province

doi:10.7668/hbnxb.2008.04.005

Abstract

Abstract: One pair of primers for amplifying the F gene of NDV on GenBank were designed,withwhich the predict - ed 1. 7 kb fragment had been amplified via RT -PCR from NDV strainHebei. The 1. 7 kb fragment was inserted into pMD - 19T vector. DNA sequence analysis showed that the cloned fragment consisted of 1662 bp and coding for 553 amino acid. Compaired with 13 strainsof NDV on GenBank, neucleitide and amino acid sequence homologieswere found to be 97. 2% - 99. 2% and 96. 9% - 98. 7% respectively, but the amino acid sequence homologies to other NDV strains such as La - Sota,F48E9 and B1 were only 88. 6% - 91. 5%. The deduced amino acid sequence of the cleavage site of F protein of the NDV isolates were 112R -R -Q -K -R -F -I -G119. the NDV isolates belonged to the genetype.

Key words: Newcastle disease virus, F gene, Cloning, Sequence analysis

CLC Number:

S432.4+1

ZHANG Li-qing, WANG Ai-hua, ZHANG Xiu-shan, PAN Li-na, DU Dong-hua, ZHOU Jing, YAN Shu-cai, SUN Ji-guo. Cloning and Sequence Analysis of F Gene of Newcastle Disease Virus Isolated from Hebei Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 19-22. doi: 10.7668/hbnxb.2008.04.005.

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