Abstract: One pair of primers was designed based on the pig IL- 2 gene sequences published in GenBank and used to amplify the interleukin-2 cDNA of Huainan Pig by RT-PCR from total RNA extracted from spleen lymphocytes,which were cultivated and stimulated with 10 μ g/mL ConA in vitro for 27 h. Then the amplified cDNA was cloned into pGEM- T Easy vector and sequenced. The result showed that the full length of Huainan pig IL- 2 was 516 bp with an ORF of 465 bp,encoding a peptide of 154 amino acids which molecular weight is 17. 4 kDa, pI 5. 27, hydrophobic amino acid 38. 3%, hydrophilic amino acid 42. 0%,basic amino acid 11%and acidic amino acid 23%. The sequence was the same as the corresponding genes published in Genebank. The base similarity of pig IL- 2 with those of cat,cattle,chicken,dog, duck,goat,horse,human and house mouse was 83. 7%,82. 6%,28. 2%,80. 6%,29. 6%,83. 4%,81. 3%,82. 0%and 61. 5%, respectively.

Key words: Procine, Interleukin -2, Gene, Cloning, Phylogenetic analysis

CLC Number: 

• Q785