Abstract: Immature cotyledons of diploid mini-watermelon inbred-line 21,90,141,149,157 were used for inducing tetraploid by tissue culture.The effects on double rate treated by different colchicine concentration,treating time and regenerate ways were studied.Results indicated that: the tissue could regenerate sigle bud,crowed buds or callus.Without colchicine inducing the tissue had high ability of regenerating buds directly.Otherwise the buds were restrict,and the numbers of calluses were more than that of buds.The tissue type and quantities were related to gene type,colchicine concentration and treating time.The calluses were easy to different plants induced by suitable colchicine concentration.To identify the plants,we got rather high propotion of tetraploid induced by 0.03% colchicine in 9 days or by 0.05% colchicine in 7 days,and high double rate by way of callus.

Key words: Mini-watermelon, Tissue culture, Induction, Tetraploid

CLC Number: 

• S651.01