Abstract: With a pair of specfric primers based on Potato Leaf Roll Virus(Pb)genomic conservative sequence lying on the both side of Intergenic Sequence(IS) reported in Genbank, one cDNA fragment(369 bp) containing the IS was amplified by reverse transcription polymerase chain reaction(RT-PCR) using the total RNA of PbV Inner Mongolia isolate as a template. The fragment was cloned into pBS-T vector and identified by PCR, restrictive enzyme analysis and nucleotide sequence analysis. The cloned IS sequence was compared with that of homologous gene of all other 13 available PbV isolates. The results showed that it had high homology with the other isolates(the highest homology reached 100% of nucleic acid and the average homology was 97. 90%, while the homology of 13 PbV complete genome was 96. 81%).The IS was not only conservative anong PbV strains but also conservative in the sequences of PbV complete genome. The result implied that transgen cplant with high resistance to PbV could be obtained through transfening RNA interfere structure of IS into potatoes.

Key words: PbV, Inner Morgolia isolate, Intergenic sequence(IS), cDNA cloning, Sequence analysis

CLC Number: 

• S432.41

• Q78