Utilization of DNA Molecular Marker Technology in the Discrimination of Peanut Cultivars

doi:10.3321/j.issn:1000-7091.2006.z2.026

Abstract

Abstract: Ten peanut cultivars from China were utilized in an attempt to evaluate the discrimination power of different molecular marker systems,including AFLP,SRAP,SSR and STS.AFLP and SRAP technology failed to identify individual peanut cultivar using a single primer pair,suggesting that more primer pairs and/or restriction enzyme combinations are needed.Nine primer pairs,viz,Lec_1,Ah4_26,Ah4_4,SsS14,SHPAL_1,PG_71,PG_43,PM_36,PG_22,resulting from screening of 103 SSR/STS primer pairs proved to have the discrimination power of 100%.Owing to the limited number of morphological,cytogenetic and biochemical markers in cultivated peanut,the 9 informative primer pairs are of significance in peanut genetic and variety identification studies.

Key words: Peanut, Variety identification, Molecular marke

CLC Number:

S565.2

WANG Chuan-tang, YANG Xin-dao, YU Xiang, ZHANG Jian-cheng, LIU Guang-zhen, XU Jian-zhi. Utilization of DNA Molecular Marker Technology in the Discrimination of Peanut Cultivars[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S2): 110-113. doi: 10.3321/j.issn:1000-7091.2006.z2.026.

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http://www.hbnxb.net/EN/Y2006/V21/IS2/110

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