Abstract: Saussurea simpsoniana was a kind of plant which was close to die out.It is an effective approach that researched and preserved the sources of species by tissue culture.In this experiment,Saussurea simpsoniana callus was induced from the hypocotyls of sterile seedling,which collected from the Pamirs in XinJiang and was cultured on solid MS medium contained 0.2(mg/L) 6-BA and 2(mg/L) NAA for 20 days.Only pink Callus had differentiation capability.Adventitious buds were induced from pink Callus,which were disposed in 4℃,on solid MS medium contained 6-BA 2(mg/L) and NAA 0.2 mg/L and 250 mg/L Lactalbumin Hydrolysate for 25 days,the buding rate was 240%.Cluster shoots were set up through subculturing.The propagation times were up to 3-5.When the shoot developed 2-3cM Length,they were cultured on MS medium supplemented with 0.5 mg/L IAA and 0.5% active carbon for 20 days.Roots were formed from cauline base,then the rate of rooting was up to 74%.

Key words: Saussurea simpsoniana, Tissue culture

CLC Number: 

• S949.783.5