The Studies on the Factors Affecting the Callus Induction and Plant Regeneration of Oat (Avena nuda L)

doi:10.3321/j.issn:1000-7091.2006.04.008

Abstract

Abstract: The influence of genotypes,medium and explant on induction,subculture and regeneration of oat callus have been studied by tissue culture of immature embryo and inflorescence of three oat varieties(line).The results showed that genetype and medium had an important role in callus induction.They were very significant and significant respectively on immature embryo and inflorescence.The formation of callus was also affected by explant and it was difference with the medium composition,inflorescence were easier to culture than immature embryo.2,4D concentration influenced callus growth and embryogenic callus generation and it can accelerate callus development and embryogenic callus generation in 3 mg/L.Callus from inflorescence of "Cao you no.1" had strong subculture capacity,it had 46.58% differentiation rate after 330 days subculture.This material had great potential in tissue culture and in genetic engineering.

Key words: Avena muda L, Immature embryo, Inflorescence, Tissue culture

CLC Number:

S512.6

JIA Li-min, FU Xiao-feng, SUN Guo-qin, SUN Rui-fen, SI Qin Bateer, LIU Li-ping, LIU Jun-qing. The Studies on the Factors Affecting the Callus Induction and Plant Regeneration of Oat (Avena nuda L)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 31-34. doi: 10.3321/j.issn:1000-7091.2006.04.008.

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