Characterizing the Genetic Diversity of a Group of Hexaploid Triticale

doi:10.3321/j.issn:1000-7091.2006.06.009

Abstract

Abstract: The genetic diversity and genetic relationships among ten accessions of Hexaploid Triticale and one accession of wheat and rye were evaluated by RAPD markers.Totally 385 bands were detected with 10 primers,with the average of 38.5.384 polymorphic bands were detected,representing of 99.74% of polymorphic bands(PPB).8 to 71 polymorphic bands were generated by each primer.Among 12 material,the RAPD-based genetic distance(RAMP-GD) ranged from 0.724 to 0.833,with a mean of 0.77.UPGMA cluster analysis showed that these accessions could be divided into three groups.The results provide a theoretical basis for transferring fine genes from Hexaploid Triticale to wheat.

Key words: Hexaploid Triticale, RAPD marker, Genetic diversity, Cluster analysis

CLC Number:

S512.103

ZHOU Fu-ping, SUN Dai-zhen, WANG Shu-guang, LI Rui, JI Cai-ping. Characterizing the Genetic Diversity of a Group of Hexaploid Triticale[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 33-36. doi: 10.3321/j.issn:1000-7091.2006.06.009.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2006.06.009

http://www.hbnxb.net/EN/Y2006/V21/I6/33

References

[1] Zillinskyfj.The development of triticale[J].Adv Agron,1974,26:315.
[2] 董卫民,王宏,李凤兰.小黑麦在畜牧业生产中的应用[J].中国草食动物,2003,(3):28-29.
[3] 孙元枢.中国小黑麦遗传育种研究和利用[M],杭州:浙江科学技术出版社,2002.
[4] 董卫民,张少敏.小黑麦的生产特性及开发利用前景[J].当代畜牧,2002,(2):33-35.
[5] Mumm R H,Dudley J W.A classification of 148 U S maize inbreds:ⅡVariation and cluster analysis based on RFLPs[J].Crop Sci,1994,4(3):842-851.
[6] 吴强,廖兰杰.野生稻基因组随机扩增DNA多态性(RAPD)分析[J].热带亚热带植物学报,1998,6(3):260-266.
[7] 倪中福,孙其信,刘志勇,等.小麦杂种优势群研究Ⅱ.普通小麦、西藏半野生小麦和斯卑尔脱小麦RAPD分子标记遗传差异研究[J].农业生物技术学报,1997,5(2):103-111.
[8] Thomson D,Henry R.Single-step protocol for preparation of plant tissue for analysis by PCR[J].Bio Techniques,1995,19:394-400.
[9] Nei M,Li W H.Mathematical model for studying genetic variation in term of restriction endonucleases[J].Proc Natl Acad Sci USA,1979,76:5269-5273.
[10] Russell J R,Fuller J D,Macaulay M,et al.Direct comparison of levelgenetic variation among barley accessions detected by RFLPs,AFLPs,SSRs and RAPDs[J].Theor Appl Genetic,1997,95:714-722.
[11] Powell W,Morgante M,Andre C,et al.The comparision of RFLP,RAPD,AFLP and SSR (microsatellite) marker for germplasm analysis[J].Mol breeding,1996,2:225-238.
[12] 孙其信,黄铁城,倪中福,等.小麦杂种优势群研究I.利用RAPD标记研究小麦品种间遗传差异[J].农业生物技术学报,1996,4(2):103-110.
[13] Sun Qixin,Ni Zhongfu,Liu Zhiyong,et al.Genetic relationships and diversity among Tibetan wheat,common wheat and European spelt wheat revealed by RAPD markers[J].Euphytica,1998,99:205-211.
[14] 贾继增,张正斌,Devos K,等.小麦21条染色体RFLP作图位点遗传多样性分析[J].中国科学C辑,2001,31(1):13-21.
[15] 杜金昆,姚颖垠,倪中福,等.普通小麦、斯卑尔脱小麦、密穗小麦和轮回选择后代材料ISSR分子标记遗传差异研究[J].遗传学报,2002,29(5):445-452.
[16] 时津霞,乔永利,杨庆文,等.野生二粒小麦(Triticum dicoccoides)与普通小麦(T.aestivum)A,B染色体组的同缘性分析[J].作物学报,2005,31(6):723-729.

Please choose a citation manager

Content to export