A New Method of Genomic DNA Extraction of Maize

doi:10.3321/j.issn:1000-7091.2006.02.003

Abstract

Abstract: A rapid and simple DNA extraction is essential in molecular maker aided selection in maize as a large amount of samples needed for analysis.In this paper,a new method of genomic DNA extraction from maize was established with guanidiniumthiocyanate (GuSCN),an indispensable reagent,combined with use of the Qiagen Tissuelyser.This method is less-reagent used,rapid and simple to run,cost-saving,etc.Three hundred DNA samples could be extracted each workday by per capita using this method,revealing that this method can be effectively used in marker analysis of maize.

Key words: Maize, DNA extraction, GuSCN, Molecular marker

CLC Number:

S513.01

LEI Kai-rong, SHI Chun-yan, LI Ming-shun, LI Xiao-hui, LI Xin-hai. A New Method of Genomic DNA Extraction of Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 10-12. doi: 10.3321/j.issn:1000-7091.2006.02.003.

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