Temperature Influence on Transformation Efficiency Agrobacterium tumefaciens

doi:10.3321/j.issn:1000-7091.2004.02.020

Abstract

Abstract: In this article,five co-cultivation temperatures (19,22,25,28 and 31 ℃) were evaluated to determine their effects on rape and maize Agrobacterium tumefaciens transformation efficiency. Rape hypocotyl and maize immature embryo explants were co-cultivated with Agrobacterium tumefaciens LBA4404 containing plas-mids encoding resistance to the herbicide phosphinothricin and chitinase gene. Explants were selected anti-cullus on medium with herbicide phosphinothricin. The results showed that, temperature has a large effects on transformation of rape and maize, Co-culture at 25 ℃ led to the highest number of stable transformed cullus. Although, there have remarkable diversity between 25 ℃ and 19 ℃, 22℃, 28℃ and 31 ℃ on-maize transformation efficiency, on rape transformation efficiency 25℃ and 22℃ have not remarkable diversity.

Key words: Temperature, Agrobacterium tumefaciens, Transformation efficiency

CLC Number:

S182

GAO Wu-jun, WEI Kai-fa, SUN Fu-cong, LU Long-dou, WANG Jing-xue. Temperature Influence on Transformation Efficiency Agrobacterium tumefaciens[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(2): 81-83. doi: 10.3321/j.issn:1000-7091.2004.02.020.

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