Enzyme Activity Assay and Fungal Resistance Evaluation of Chitinase Expressing Tobacco

doi:10.3321/j.issn:1000-7091.2002.04.001

Abstract

Abstract: A fluorometric assay was used to confirm enzyme activity in chitinase gene transferred tobacco. Chitinase gene activity in transgene tobacco is higher than that in untransgene counterparts. Confocal laser scanning microscopy of foliar sect ions f rom a chitinase expressing Nicotiana tabacum Xanthi- nc embedded in ( butyl- methyl methacrylate) and stained with a polyclonal serum raised against the chit inase/ fluor-conjugated secondary (IgG) antibody detection system showed that chit inase was largely rest ricted to the vascular tissue, with a few accumulating in chloroplasts. Among the transgenic tobacco T₂ plants inoculated with Colletotrichum nicotianae Averna, several lines displayed resistance to the fungus.

Key words: Tobacco, Chitinase gene, Enzyme act ivity, Fungal diseases resistance evaluation

CLC Number:

S572

SHI Jiao, HAN Jin-feng, XU Yi-min, Cooper JI, LIU Hua-shan. Enzyme Activity Assay and Fungal Resistance Evaluation of Chitinase Expressing Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 1-4. doi: 10.3321/j.issn:1000-7091.2002.04.001.

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