Detection of Activities of Toxins from Pyricularia oryzae and Conditions Affecting the Activities

doi:10.3321/j.issn:1000-7091.1998.03.008

Abstract

Abstract: Pyricularia oryzae 90 2 strain which is isolated from rice CMS in field is selectively pathogenic to rice W CMS. The strain produces toxin that plays an important role in pathogenic process. This paper reports heat stability of the toxin and effects of pH on toxin production. The experiments show that toxin from 90 2 strain is heat stable and variations of pH value to certain extent affects the activities of toxin. The change of pH value of medium also alters the activities of the toxin. We observed that the toxin lead to the yellowing of leaves and cell necrosis. The degree of yellowing of W Zhenshan 97A leaves is larger than that of Zhenshan 97B. The experiments detected the influence of the toxin on total content of chlorophyll and respiration of W Zhenshan 97A and 97B respectively. In addition, we detected the pathogenicity of the toxin to one set of rice materials with homocaryon using the method of root cap cells bioassay. The experiment confirms that 90 2 strain is special susceptibility to rice with W CMS.

Key words: Rice, Pyricularia oryzae, Toxin, Male sterile cytoplasm

CLC Number:

S435.11

Yan Zhifen， Zhang Hongxin， Cui Siping， Wang Lian， Wei Jiankun. Detection of Activities of Toxins from Pyricularia oryzae and Conditions Affecting the Activities[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(3): 41-46. doi: 10.3321/j.issn:1000-7091.1998.03.008.

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