Effects of Exogenous Trehalose on Physiological Characteristics and Gene Expression in Aromatic Rice Seedlings under Drought Stress

doi:10.7668/hbnxb.20196434

Abstract

Abstract:

To investigate the mechanism of exogenous trehalose in mitigating drought stress in aromatic rice,this study used Daohuaxiang 2 as experimental material and simulated drought stress with 15% polyethylene glycol-6000(PEG-6000).Three trehalose spray treatments were applied at concentrations of 5,10,and 15 mmol/L(T1,T2,T3),with well-watered(CK,sprayed with water)and drought-stressed(D,sprayed with water)conditions as controls.Temporal changes in growth and physiological parameters of seedlings were systematically analyzed and combined with transcriptome sequencing to elucidate the molecular regulatory mechanisms.The results showed that the 5 mmol/L trehalose treatment(T1)exhibited a significant alleviating effect during the late stress stage(12 d),with soluble protein and proline contents in leaves reaching 5.47 and 0.132 mg/g,respectively,significantly higher than those in the D treatment.Under the T₁ treatment,the ascorbic acid(AsA)content and the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and ascorbate peroxidase(APX)peaked,with SOD and CAT activities significantly increased by 30.10% and 91.84%,respectively,compared with the D treatment.In contrast,the superoxide anion production rate and malondialdehyde(MDA)content were significantly lower in T1 than in D,with reductions of 55.10% and 14.68%,respectively.A Z-score comprehensive evaluation based on morphological and physiological parameters revealed that the T1 treatment had the highest comprehensive evaluation value of 455.18,which was 184.65% and 44.02% higher than that of D and CK,respectively.Transcriptome analysis indicated that the T1 treatment regulated 1 451 differentially expressed genes,which were significantly enriched in pathways such as photosynthesis-antenna proteins,glutathione metabolism,and MAPK signaling pathways.Key genes including OsFPPS,OsGSTF1,OsTPP1,and OsWRKY69 were upregulated,while negative regulators such as OsPP2C73 were downregulated.In conclusion,exogenous application of 5 mmol/L trehalose effectively alleviated drought stress in Daohuaxiang 2 seedlings by enhancing osmotic adjustment and antioxidant capacity,while coordinately regulating ABA signaling,MAPK signaling pathway,and endogenous trehalose metabolism-related genes.These findings provide a theoretical basis and molecular targets for drought-resistant cultivation of aromatic rice and the development of trehalose-based stress resistance agents.

Key words: Aromatic rice, Drought stress, Trehalose, Physiological mechanisms, Transcriptomic analysis

CLC Number:

GUO Yukun, ZHAO Jingyi, BI Yanpeng, MIAO Yuhang, PENG Xinyi, WANG Haozhou, HOU Haoran, ZHANG Zewei, WANG Jiameng, GUO Xiaohong. Effects of Exogenous Trehalose on Physiological Characteristics and Gene Expression in Aromatic Rice Seedlings under Drought Stress[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(5): 11-22. doi: 10.7668/hbnxb.20196434.

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References 23

[1]	朱子杰, 卜柳冰, 王雨鑫, 徐佳婷, 刘丹彤, 缪军. 2C类蛋白磷酸酶基因OsPP2C49调控水稻干旱和高温胁迫响应的机制研究[J]. 扬州大学学报(农业与生命科学版), 2024, 45(3):17-27.doi:10.16872/j.cnki.1671-4652.2024.03.002.
	Zhu Z J, Bu L B, Wang Y X, Xu J T, Liu D T, Miao J. Mechanism of the rice type 2C protein phosphatase gene OsPP2C49 in regulating drought and high-temperature stress responses[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2024, 45(3):17-27.
[2]	魏晓东, 张亚东, 赵凌, 路凯, 宋雪梅, 王才林. 稻米香味物质2-乙酰-1-吡咯啉的形成及其影响因素[J]. 中国水稻科学, 2022, 36(2):131-138.doi:10.16819/j.1001-7216.2022.201214.
	Wei X D, Zhang Y D, Zhao L, Lu K, Song X M, Wang C L. Research progress in biosynthesis and influencing factors of 2-acetyl-1-pyrroline in fragrant rice[J]. Chinese Journal of Rice Science, 2022, 36(2):131-138. doi: 10.16819/j.1001-7216.2022.201214
[3]	Mondal K, Singh R K, Dey N. Combined drought and heat stress affect starch synthesis and grain quality-related traits during the grain-filling stage of rice(Oryza sativa L.)[J]. Journal of the Science of Food and Agriculture, 2025, 105(15):8511-8524.doi:10.1002/jsfa.70096. URL
[4]	Yu Q T, Li Z, Liu N X. Causes and countermeasures for fragrance diminishing of rice cultivar Daohuaxiang 2 in Heilongjiang Province[J]. Agricultural Science & Technology, 2016, 17(10):2293-2296.doi:10.16175/j.cnki.1009-4229.2016.10.018.
[5]	张旭斌. 外源海藻糖和赤霉素对干旱胁迫下小麦生长的效应分析及66份高代系苗期抗旱性评价[D]. 咸阳: 西北农林科技大学, 2025.doi:10.27409/d.cnki.gxbnu.2025.000961.
	Zhang X B. Effects of exogenous trehalose and gibberellin on wheat growth under drought stress and evaluation of drought resistance of 66 high-generation lines at seedling stage[D]. Xianyang: Northwest A&F University, 2025.
[6]	李宇星, 马亮亮, 张月, 秦博雅, 张文静, 马尚宇, 黄正来, 樊永惠. 外源海藻糖对灌浆期高温胁迫下小麦旗叶生理特性和产量的影响[J]. 作物学报, 2023, 49(8):2210-2224.doi:10.3724/SP.J.1006.2023.21057.
	Li Y X, Ma L L, Zhang Y, Qin B Y, Zhang W J, Ma S Y, Huang Z L, Fan Y H. Effect analysis of exogenous trehalose on physiological characteristics and yield of wheat flag leaves under high temperature stress at grain filling stage[J]. Acta Agronomica Sinica, 2023, 49(8):2210-2224. doi: 10.3724/SP.J.1006.2023.21057 URL
[7]	李佳馨, 谢寅峰, 李霞, 王净. 海藻糖对转C4型PEPC水稻种子萌发耐旱性的影响[J]. 核农学报, 2021, 35(12):2879-2892.doi:10.11869/j.issn.100-8551.2021.12.2879.
	Li J X, Xie Y F, Li X, Wang J. Effects of trehalose on seed germination of C4-PEPC transgenic rice for drought tolerance[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(12):2879-2892.
[8]	黄亚茹, 徐鹏, 王乐乐, 贺一哲, 王辉, 柯健, 何海兵, 武立权, 尤翠翠. 外源海藻糖对粳稻品系W1844籽粒灌浆特性及产量形成的影响[J]. 中国水稻科学, 2023, 37(4):379-391.doi:10.16819/j.1001-7216.2023.220712.
	Huang Y R, Xu P, Wang L L, He Y Z, Wang H, Ke J, He H B, Wu L Q, You C C. Effects of exogenous trehalose on grain filling characteristics and yield formation of japonica rice cultivar W1844[J]. Chinese Journal of Rice Science, 2023, 37(4):379-391.
[9]	徐鹏, 贺一哲, 方双文, 朱铁忠, 武立权, 尤翠翠. 外源海藻糖对高温胁迫下穗分化期水稻叶片生理特性及产量的影响[J]. 西北植物学报, 2023, 43(4):628-637.doi:10.7606/j.issn.1000-4025.2023.04.0628.
	Xu P, He Y Z, Fang S W, Zhu T Z, Wu L Q, You C C. Effects of exogenous trehalose on leaf physiological characteristics and yield of rice at panicle initiation stage under high temperature stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2023, 43(4):628-637.
[10]	郭宇坤, 张燕飞, 野金花, 李佳擎, 金智慧, 苗宇航, 吕艳东, 郭晓红. 不同氮用量对香稻幼苗生长及生理指标的影响[J]. 山东农业科学, 2024, 56(3):78-85.doi:10.14083/j.issn.1001-4942.2024.03.010.
	Guo Y K, Zhang Y F, Ye J H, Li J Q, Jin Z H, Miao Y H, Lyu Y D, Guo X H. Effects of different nitrogen amounts on growth and physiological indexes of fragrant rice seedlings[J]. Shandong Agricultural Sciences, 2024, 56(3):78-85.
[11]	邓丽莉, 潘晓倩, 生吉萍, 申琳. 考马斯亮蓝法测定苹果组织微量可溶性蛋白含量的条件优化[J]. 食品科学, 2012, 33(24):185-189.doi:10.7506/spkx1002-6630-201224038.
	Deng L L, Pan X Q, Sheng J P, Shen L. Optimization of experimental conditions for the determination of water soluble protein in apple pulp using coomassie brilliant blue method[J]. Food Science, 2012, 33(24):185-189. doi: 10.7506/spkx1002-6630-201224038
[12]	职明星, 李秀菊. 脯氨酸测定方法的改进[J]. 河南科技学院学报(自然科学版), 2005, 33(4):10-12.doi:10.3969/j.issn.1673-6060-B.2005.04.003.
	Zhi M X, Li X J. Improvement of the method for measuring proline content[J]. Henan Institute of Science and Technology(Natural Science Edition), 2005, 33(4):10-12.
[13]	孙联合, 李艳, 赵红星, 王梦扬. 茄果类蔬菜抗坏血酸含量测定方法[J]. 种业导刊, 2024(5):10-12.doi:10.3969/j.issn.1003-4749.2024.05.003.
	Sun L H, Li Y, Zhao H X, Wang M Y. Method for determination of ascorbic acid content in Solanaceous vegetables[J]. Journal of Seed Industry Guide, 2024(5):10-12.
[14]	沈文飚, 徐朗莱, 叶茂炳, 张荣铣. 抗坏血酸过氧化物酶活性测定的探讨[J]. 植物生理学通讯, 1996, 32(3):203-205.doi:10.13592/j.cnki.ppj.1996.03.016.
	Shen W B, Xu L L, Ye M B, Zhang R X. Discussion on determination of ascorbic acid peroxidase activity[J]. Plant Physiology Communications, 1996, 32(3):203-205.
[15]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	Li H S. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing: Higher Education Press, 2000.
[16]	Ahmad S, Aftab M N, Aslam M S, Rehman A U, Haq I U, Ali S, Usman M. Trehalose-abscisic acid pathway feedback loops in wheat-Piriformospora indica symbiosis:mechanisms and drought resilience[J]. The Microbe, 2025, 9:100561.doi:10.1016/j.microb.2025.100561. URL
[17]	张学彪, 倪忠, 庄义庆. 外源海藻糖对NaCl胁迫下大豆幼苗的影响[J]. 江苏农业科学, 2024, 52(21):116-122.doi:10.15889/j.issn.1002-1302.2024.21.015.
	Zhang X B, Ni Z, Zhuang Y Q. Effects of exogenous trehalose on soybean seedlings under NaCl stress[J]. Jiangsu Agricultural Sciences, 2024, 52(21):116-122.
[18]	吴宪, 李洪鑫, 卢宗志. 玉米苗期谷胱甘肽S-转移酶活性与温度、降雨的相关性研究[J]. 玉米科学, 2020, 28(4):105-108.doi:10.13597/j.cnki.maize.science.20200416.
	Wu X, Li H X, Lu Z Z. Study on relationship between glutathione S-transferase,low temperature,and rainfall in maize seeding[J]. Journal of Maize Sciences, 2020, 28(4):105-108.
[19]	王文霞, 刘宇琪, 夏清, 智慧, 杜杰. 外源硒对干旱胁迫下胡麻生理特性的影响[J]. 河南农业科学, 2025, 54(7):55-63.doi:10.15933/j.cnki.1004-3268.2025.07.006.
	Wang W X, Liu Y Q, Xia Q, Zhi H, Du J. The impact of exogenous selenium on the physiological characteristics of flax under drought stress[J]. Journal of Henan Agricultural Sciences, 2025, 54(7):55-63. doi: 10.15933/j.cnki.1004-3268.2025.07.006
[20]	叶玉秀, 陆大雷, 王飞兵, 陈新红, 戚名扬, 何润, 陆卫平. 干旱胁迫下外源海藻糖对糯玉米幼苗生理特性的影响[J]. 玉米科学, 2020, 28(3):80-86.doi:10.13597/j.cnki.maize.science.20200310.
	Ye Y X, Lu D L, Wang F B, Chen X H, Qi M Y, He R, Lu W P. Effects of exogenous trehalose on physiological characteristics in waxy maize seedlings under drought stress[J]. Journal of Maize Sciences, 2020, 28(3):80-86.
[21]	Huang P P, Niu K J, Chai J K, Wang W P, Ma Y M, Cao Y N, Zhao G Q. Genome-wide identification of PP2C gene family in oat(Avena sativa L.) and its functional analyses in response to ABA and abiotic stresses[J]. Plants, 2025, 14(13):2062.doi:10.3390/plants14132062.
[22]	Zhu Y F, Li Z B, Wang W J, Liu X J, Xie Q X, Yu X L, Zhang X C, Li S X, Ruan M B. MeWRKY30,a cassava stress-responsive WRKY transcription factor,confers drought resistance to transgenic Arabidopsis[J]. Plant Cell Reports, 2025, 44(7):153.doi:10.1007/s00299-025-03543-5.
[23]	欧晋稳, 张古文, 冯志娟, 王斌, 卜远鹏, 徐钰, 茹磊, 刘娜, 龚亚明. 大豆海藻糖-6-磷酸磷酸酶基因GmTPP的鉴定及其在生长发育和非生物胁迫响应中的表达分析[J]. 浙江农业学报, 2024, 36(9):2031-2041.doi:10.3969/j.issn.1004-1524.20231003.
	Ou J W, Zhang G W, Feng Z J, Wang B, Bu Y P, Xu Y, Ru L, Liu N, Gong Y M. Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9):2031-2041.

基因名称 Gene name	基因登录号 Gene ID	正向引物序列(5'―3') Forward primer sequence(5'―3')	反向引物序列(5'―3') Reverse primer sequence(5'―3')
OseIF(内参)	LOC4343445	CATGATCACCGGTACCTCG	CCAGCATGTTGTCTCCGTG
OsTPS13	LOC9270657	CTTAGCTGCATCACACAGTGG	ATTCCAGCGATCTTCGCCTC
OsLAC15	LOC4348374	CAGGGATGAGGGCAAGTTCA	TTAACCATGCCCCTGGGTTG
OsPRPs	LOC4341639	TCTAGTTCAGCCGCCATTCC	CGAGATCGAGTAGCCAACAAAC
OsPRX2	LOC4327090	GACTGCTTCGTCAACGGGT	TCTTCTCCCCGGTGAAGTTCG
OsPP2C73	LOC4349568	AGGAGCTTGAGGAGGAGGG	CCCGCAAAGTCCTGGCA
OsPP2C	LOC4351280	ACCAAATCTTCCAAGCGAAGC	CTGGTAGCCACATCCTAGACAC
OsTDR	LOC4349271	CCTCCTGCCTACAGGATGAC	CATCTCCGTAGCCTGCACTC
OsbHLH6	LOC4335417	CCTCTTCGTCGAGGGTGTG	TATCGGACATTGACGACGGC
OsWRKY69	LOC9270274	TCCCCAAGAGAAGGAGGCA	TTCCTCCAGGACCAACTGTC
OsbHLH96	LOC9267196	CTACGTCCAGAGGGGCGAC	GCATCTCGCCTTGTGGTGA

基因名称 Gene name	基因登录号 Gene ID	正向引物序列(5'―3') Forward primer sequence(5'―3')	反向引物序列(5'―3') Reverse primer sequence(5'―3')
OseIF(内参)	LOC4343445	CATGATCACCGGTACCTCG	CCAGCATGTTGTCTCCGTG
OsTPS13	LOC9270657	CTTAGCTGCATCACACAGTGG	ATTCCAGCGATCTTCGCCTC
OsLAC15	LOC4348374	CAGGGATGAGGGCAAGTTCA	TTAACCATGCCCCTGGGTTG
OsPRPs	LOC4341639	TCTAGTTCAGCCGCCATTCC	CGAGATCGAGTAGCCAACAAAC
OsPRX2	LOC4327090	GACTGCTTCGTCAACGGGT	TCTTCTCCCCGGTGAAGTTCG
OsPP2C73	LOC4349568	AGGAGCTTGAGGAGGAGGG	CCCGCAAAGTCCTGGCA
OsPP2C	LOC4351280	ACCAAATCTTCCAAGCGAAGC	CTGGTAGCCACATCCTAGACAC
OsTDR	LOC4349271	CCTCCTGCCTACAGGATGAC	CATCTCCGTAGCCTGCACTC
OsbHLH6	LOC4335417	CCTCTTCGTCGAGGGTGTG	TATCGGACATTGACGACGGC
OsWRKY69	LOC9270274	TCCCCAAGAGAAGGAGGCA	TTCCTCCAGGACCAACTGTC
OsbHLH96	LOC9267196	CTACGTCCAGAGGGGCGAC	GCATCTCGCCTTGTGGTGA

处理 Treatment	株高 Plant height	地上部鲜质量 Shoot fresh weight	地下部鲜质量 Root fresh weight	根冠比 Root/ shoot ratio	根系总长 Total root length	根系投影面积 Root proje- cted area	根表面积 Root surface area	根体积 Root volume	根平均直径 Mean root diameter
海藻糖浓度	-0.828^**	-0.886^**	-0.237	0.886^**	-0.835^**	-0.601	-0.712^*	-0.547	-0.780^*
Trehalose concentration
处理 Treatment	可溶性蛋白含量 Soluble protein content	Pro	AsA	POD	SOD	CAT	APX	O₂^-	MDA
海藻糖浓度	-0.849^**	-0.984^**	-0.721^*	-0.949^**	-0.973^**	-0.870^**	-0.799^**	0.787^*	0.968^**
Trehalose concentration

处理 Treatment	株高 Plant height	地上部鲜质量 Shoot fresh weight	地下部鲜质量 Root fresh weight	根冠比 Root/ shoot ratio	根系总长 Total root length	根系投影面积 Root proje- cted area	根表面积 Root surface area	根体积 Root volume	根平均直径 Mean root diameter
海藻糖浓度	-0.828^**	-0.886^**	-0.237	0.886^**	-0.835^**	-0.601	-0.712^*	-0.547	-0.780^*
Trehalose concentration
处理 Treatment	可溶性蛋白含量 Soluble protein content	Pro	AsA	POD	SOD	CAT	APX	O₂^-	MDA
海藻糖浓度	-0.849^**	-0.984^**	-0.721^*	-0.949^**	-0.973^**	-0.870^**	-0.799^**	0.787^*	0.968^**
Trehalose concentration

处理 Treatment	各指标的综合评价值 Comprehensive evaluation value of each indicator															∑Z_i
处理 Treatment	株高 Plant height	地上部鲜质量 Shoot fresh weight	根冠比 Root/shoot ratio	根系总长 Total root length	根表面积 Root surface area	根平均直径 Mean root diameter	可溶性蛋白含量 Soluble protein content	Pro	AsA	POD	SOD	CAT	APX	O₂^-	MDA	∑Z_i
CK	21.03	15.75	26.19	56.64	87.68	129.26	129.25	13.87	7.26	133.59	55.17	51.27	56.87	55.96	411.81	316.05
D	14.06	9.37	26.46	46.97	74.93	131.75	164.90	30.76	14.45	144.01	69.40	71.80	84.16	97.61	625.49	159.91
T1	18.55	13.96	26.24	65.83	100.93	137.75	181.93	55.10	24.33	155.42	82.14	103.07	119.60	72.05	557.62	455.18
T2	15.24	11.81	27.34	59.31	96.20	144.14	167.89	43.80	25.53	155.00	76.08	88.76	105.25	87.44	568.12	360.78
T3	14.23	11.11	27.98	53.66	84.88	130.87	163.90	36.60	17.38	147.54	69.68	84.09	100.47	92.88	597.79	251.75

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