Effect of Aspergillus niger on Seed Germination and Seedling Growth of Soybean

doi:10.7668/hbnxb.20192921

Abstract

Abstract:

In order to explore the effect of phosphorus solubilizing and potassium solubilizing fungus Aspergillus niger Z8 on soybean seed germination and seedling growth,the biomass,physiological effects,soil enzyme activity and soil microbial changes of soybean seeds and seedlings after inoculation with Aspergillus niger Z8 were measured.The results showed that Aspergillus niger Z8 had no significant effect on the germination rate of soybean seeds,but could increase the biomass of soybean sprouts,and the overall effect of K3 treatment group was better;when the concentration of bacterial solution was 1.0%,the plant height,fresh weight,dry weight and root length increased,and the content of photosynthetic pigment in soybean seedling leaves was higher.At this time,the cell volume in root tip elongation zone increased,indicating that it had the best growth potential;the results of pot experiment showed that inoculating Aspergillus niger Z8 could significantly increase the plant height(19.44%-33.47%),root length(19.95%-33.50%),fresh weight(26.67%-86.67%),dry weight(25.00%-87.50%),soluble sugar(31.67%-47.83%),soluble protein(21.70%-35.86%).The inoculation of different treatments can increase the chlorophyll (T4>T3>T2>T1>CK), superoxide dismutase (T4>T3>T1>T2>CK),catalase(T4>T3>T2>T1>CK)and peroxidase(T4>T2>T3>T1>CK)were higher than those in the control group. At the same time, soil sucrase (T4>T3>T2>T1>CK), urease(T4>T3>T2>T1>CK),catalase(T4>T2>T3>T1>CK), acid phosphatase activity (T2>T1>T3>T4>CK), neutral phosphatase(T2>T4>T1>T3>CK)and alkaline phosphatase (T4>T3>T2>T1>CK)were increased content.It had a significant promoting effect on the number of soil microorganisms,increasing the number of bacteria by 6.03%-32.66%,fungi by 22.22%-62.22% and actinomycetes by 0.67%-20.32%.In conclusion,T4 treatment group had the best promoting effect on soybean growth.Aspergillus niger Z8 had good promoting function,which can be used as a biological fertilizer for soybean planting,and is of great significance for reducing the application of chemical fertilizer and sustainable agriculture development.

Key words: Soybean, Aspergillus niger, Growth promoting effect, Capsule

WANG Zixuan, WANG Jihua. Effect of Aspergillus niger on Seed Germination and Seedling Growth of Soybean[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 133-140. doi: 10.7668/hbnxb.20192921.

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References 30

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处理 Treatment	发芽率/% Germination percentage	芽质量/g Bud weight	芽长/mm Bud length	侧根数/支 Number of lateral roots	平均侧根长/mm Average lateral root length	总侧根长/mm Total lateral root length
CK	93.33a	1.96±0.10c	27.75±2.35d	7.93±0.86b	3.22±0.40b	43.30±7.12c
K1	92.22a	2.02±0.12b	37.30±1.14c	6.59±0.81d	3.21±0.39b	45.40±9.64b
K2	92.22a	2.11±0.19a	48.69±3.80a	7.67±0.86c	6.82±0.66b	127.69±16.62a
K3	93.33a	2.12±0.13a	41.79±1.85b	9.53±0.10a	10.34±0.85a	127.88±19.73a

处理 Treatment	发芽率/% Germination percentage	芽质量/g Bud weight	芽长/mm Bud length	侧根数/支 Number of lateral roots	平均侧根长/mm Average lateral root length	总侧根长/mm Total lateral root length
CK	93.33a	1.96±0.10c	27.75±2.35d	7.93±0.86b	3.22±0.40b	43.30±7.12c
K1	92.22a	2.02±0.12b	37.30±1.14c	6.59±0.81d	3.21±0.39b	45.40±9.64b
K2	92.22a	2.11±0.19a	48.69±3.80a	7.67±0.86c	6.82±0.66b	127.69±16.62a
K3	93.33a	2.12±0.13a	41.79±1.85b	9.53±0.10a	10.34±0.85a	127.88±19.73a

处理 Treatment	株高/cm Plant height	鲜质量/g Fresh weight	干质量/g Dry weight	根长/cm Root length
CK	20.80±1.71d	1.29±0.32d	0.09±0.01c	10.10±1.25b
0.5%	23.77±3.78b	1.53±0.10b	0.10±0.01b	10.67±0.66a
1.0%	24.43±1.83a	1.75±0.06a	0.13±0.01a	10.70±1.80a
2.5%	24.43±0.85a	1.50±0.22b	0.10±0.02b	10.28±0.49b
5.0%	22.90±0.45b	1.45±0.12c	0.11±0.01b	9.13±1.38c
10.0%	21.20±4.54c	1.43±0.02c	0.11±0.01b	8.33±0.47c

处理 Treatment	株高/cm Plant height	鲜质量/g Fresh weight	干质量/g Dry weight	根长/cm Root length
CK	20.80±1.71d	1.29±0.32d	0.09±0.01c	10.10±1.25b
0.5%	23.77±3.78b	1.53±0.10b	0.10±0.01b	10.67±0.66a
1.0%	24.43±1.83a	1.75±0.06a	0.13±0.01a	10.70±1.80a
2.5%	24.43±0.85a	1.50±0.22b	0.10±0.02b	10.28±0.49b
5.0%	22.90±0.45b	1.45±0.12c	0.11±0.01b	9.13±1.38c
10.0%	21.20±4.54c	1.43±0.02c	0.11±0.01b	8.33±0.47c

处理 Treatment	株高/cm Shoot height	根长/cm Root length	鲜质量/g Fresh weight	干质量/g Dry weight	可溶性糖/(mg/g) Soluble sugar content	可溶性蛋白/(mg/g) Soluble protein content
CK	40.63±1.16e	8.12±0.23d	2.70±0.57e	0.24±0.11e	6.00±0.22e	5.41±0.62d
T1	48.53±4.96d	9.74±1.74c	3.42±1.42d	0.30±0.11d	7.90±0.81d	6.53±0.39c
T2	52.17±5.02b	10.36±1.20b	3.63±0.25c	0.33±0.06c	8.22±0.63c	6.59±0.54b
T3	50.30±5.23c	10.39±1.03b	4.51±1.14b	0.36±0.05b	8.56±0.36b	6.57±0.20b
T4	54.23±4.02a	10.84±0.80a	5.04±0.74a	0.45±0.02a	8.87±0.31a	7.35±0.05a

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