Cloning and Expression Analysis of Transcription Factor Tamyb59 in Wheat

doi:10.7668/hbnxb.2018.06.001

Abstract

Abstract: In order to further excavation of the role of wheat stress response genes in plant abiotic stress response,explored the regulation mechanism of abiotic stress response, a MYB gene was screened from a transcriptome result and named Tamyb59, temporarily.The full length sequence of Tamyb59 was cloned by PCR. Sequence comparison and conserved domain were analyzed by NCBI and DNAMAN. Amino acid composition and hydrophilic coefficient were analyzed by online software Expasy and TMHMM. Phylogenic tree was constructed by MEGA 6.0 according to the NJ method. And a fusion expression vector pMDC83-GFP was constructed to identify the subcellular localization. The Real-time PCR (qRT-PCR) was used to analyze the expression characteristics of different tissues in different abiotic stress treatments. The significance was analyzed by SAS. The results showed that:Tamyb59 gene contained a typical conserved SANT domain. The full-length CDS of Tamyb59 was 522 bp,which encoded 173 amino acids with a molecular weight of 19.7 ku and the theoretical isoelectric point was 7.61;Phylogenetic analysis showed that Tamyb59 had 52.0%-85.6% homology with MYB transcription factors of 9 other plants (Aegilops tauschii, Oryza sativa Japonica and Zea may, etc.). Tamyb59 had the highest homology with the MYB sequence of Setaria italica. The protein of Tamyb59 encoded specifically located in the nucleus.qRT-PCR was used to analyze the expression characteristics of different tissues in different abiotic stress treatments. The results revealed that the expression level of Tamyb59 was the highest in the root, and lower in stem, leaf and young spike. Under the PEG and NaCl treatment stress, the expression of Tamyb59 showed a trend of rising first and then decreasing, indicating that Tamyb59 had different responses under different stresses.

Key words: Wheat, Tamyb59 gene, Gene cloning, Abiotic stress, Expression analysis

CLC Number:

Q78
S512.03

ZHANG Pengyu, LIU Yuxia, CAO Liru, YUAN Zhen, WANG Guorui, WANG Tongchao, YIN Jun, WEI Li. Cloning and Expression Analysis of Transcription Factor Tamyb59 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 1-7. doi: 10.7668/hbnxb.2018.06.001.

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