Genome-wide Identification and Expression Analysis of bZIP Transcription Factor Family in Jatropha curcas

doi:10.7668/hbnxb.201750960

Abstract

Abstract: The purpose of the study was to further understand the structure characteristics of Basic leucine zipper (bZIPs) and its function in response to biotic and abiotic stress. Based on the Jatropha curcas genome and bioinformatics methods, bZIP family genes from J.curcas were identified, and then the gene structure, phylogentic relationship, chromosomal location, synteny relationship, as well as organ and chilling hardening expression, were systematically analyzed. The results showed that a total of 51 bZIP genes were systematically identified from J.curcas genome and classified into 10 subfamilies (A-I and S) according to phylogentic relationship. Chromosome mapping analysis indicated that J.curcas bZIP genes were distributed with different densities on 11 chromosomes, and tandem duplications were found on chromosome No.2 and 4, which was the main power for the expansion of J.curcas bZIP gene family. The results of gene structure analysis revealed that most of the J.curcas bZIP gene contained 1-13 exons, and the member of subfamilies of S and G owned 1-2 and 12-13 exons, respectively. Subcellular localization showed that the predicted bZIP proteins mainly located in nucleus with amino acid number ranged from 113 to 768 aa and pI value distributed from 4.70 to 10.30. 3-27 hormonal response elements for gibberellin, abscisic acid, ethylene, auxin and abiotic stress response elements like low temperature, heat and wound were identified in the promoter of J.curcas bZIP genes. Transcriptome expression analysis showed that J.curcas bZIP genes exhibited different tissue-specific expression patterns, and 26 J.curcas bZIP genes were found to be expressed in the detected organs of leaves, roots, and seeds, while the others were expressed in the specific organ tissues. Moreover, the expression profiling of 14 J.curcas bZIP genes were up-regulated under chilling hardening. In leaves, JcbZIP3 and JcbZIP14 reached to the highest expression levels after 24 h chilling hardening based on the qRT-PCR results, which were associated with the events of chilling resistance formation and cold signalling transduction. These results were helpful for the cloning and regulation mechanism analysis of bZIP genes in J.curcas.

Key words: Jatropha curcas, Transcription factor, bZIP, Gene family, Expression analysis

CLC Number:

Q78
S794.03

WANG Haibo, GUO Junyun, TANG Lizhou, LIU Chao. Genome-wide Identification and Expression Analysis of bZIP Transcription Factor Family in Jatropha curcas[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 44-58. doi: 10.7668/hbnxb.201750960.

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