Construction of an SSR-based Genetic Linkage Map of Diploid Potato

doi:10.7668/hbnxb.2017.05.018

Abstract

Abstract: In order to enrich molecular markers and position the bacterial wilt resistant gene loci in potato,an SSR-based genetic linkage map was constructed from a diploid population consisting of 123 individuals derived from a cross between two potato diploid clones (C and E) which harborer resistance to Ralstonia solanacearum in this study.A total of 864 simple sequence repeat primer pairs were designed according to the potato genome sequence and used to screen parents C and E for polymorphisms.Of 187 polymorphic loci,72.2% showed clear amplifiable and scorable bands in the F₁ population.Then a total of 135 locus were mapped to 12 linkage groups,of which the cumulative length was 948.4 cM,with an average marker interval of 7.03 cM,and 5 segregation distortion regions in three linkage groups.This study provided a set of new SSR markers for genetic map construction and increased saturation of map in potato.The relatively high density map may provide a useful reference for further identification,mapping of quantitative trait loci (QTL) and cloning of potato bacterial wilt resistance genes and marker-assisted breeding.

Key words: Diploid, Potato, Simple sequence repeat, Linkage map

CLC Number:

S532.03

ZHU Yun, GUO Xiao, SUN Xiaolei, MA Weiqing, DONG Daofeng, GUO Baotai, YANG Yu, YANG Xiaohui, LI Guangcun. Construction of an SSR-based Genetic Linkage Map of Diploid Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 117-123. doi: 10.7668/hbnxb.2017.05.018.

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