Transcriptome Analysis of Superior Strains of Rhododendron Hybrids under Low Temperature Stress

doi:10.7668/hbnxb.2017.05.009

Abstract

Abstract: To explore the molecular mechanism of cold resistance of Rhododendron,screening of cold resistant genes,using artificial low temperature,excellent strains with Rhododendron Fanjing-6℃(T)and 25℃(CK)control as test materials,conduct transcriptome sequencing and de novo assembly data and bioinformatics analysis,the data obtained by assembling 122 654 Unigenes sequences,functional annotation showed that a large number of Unigenes could be annotated with GO and COG database,information,some of Unigenes,might be the unique gene sequence of Rhododendron. Rhododendron Fanjing transcription in the Unigenes group according to the GO function can be divided into cellular component,molecular function and biological process of 3 kinds of 51 branches,including Unigenes and metabolic process,a large number of catalytic activity and cell process. There were 6 347 up-regulated differentially expressed genes and down regulated expression genes of 4 482. The expression of 24 genes in the four transcription factors,with 12 up-regulated and 12 down-regulated at the transcription level,which were significantly different in the transcriptional results,was verified by the expression level under low temperature stress. It was found that the transcriptional data were reliable.

Key words: Rhododendron, Low temperature stress, Transcriptome, Cold-resistant genes

CLC Number:

S685.21
Q78

WU Yueyan, MING Meng, HE Jingwen, LU Dan, SHEN Zili, QIU Tian, WU Yanyan, XIE Xiaohong. Transcriptome Analysis of Superior Strains of Rhododendron Hybrids under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 52-60. doi: 10.7668/hbnxb.2017.05.009.

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