Cloning and Expression Characteristics of RhMYB61 Gene from Rosa hybrida

doi:10.7668/hbnxb.2017.05.010

Abstract

Abstract: In order to determine the molecular characterization and expression patterns of MYB transcription factors involved in flower opening in Rose. According to the expressed sequence tags from transcriptome assembly and RACE PCR methods,we obtained a new MYB transcription factor gene,and named RhMYB61 (GenBank accession number is KY921844),in Rosa hybrida Samantha petals. The ORF of RhMYB61 was 1 323 bp,which encoded 441 amino acids. Bioinformatics analysis showed that the molecular weight and theoretical isoelectric point of RhMYB61 was 49.1 kDa and 7.66,respectively,and the formula of RhMYB61 was C₂₁₂₃H₃₂₈₀N₆₂₀O₆₈₈S₁₈. Phylogenic tree analysis showed that RhMYB61 was clustered with PpMYB86 and EjMYB8,and belonged to the R2R3-MYB type transcription factor. Multi-alignment of RhMYB61 of several plants found that RhMYB61 had the seven characteristic conservative motifs.Two conserved domains,including R2-MYB domain and R3-MYB domain,were found in its N-terminal. The nuclear localization sites was found in R3-MYB domain. Qualitative PCR analysis showed that RhMYB61 expression was induced as the rose flower opening. RhMYB61 expression was accumulated at 12 h dehydration treatment,compared with control. In addition, RhMYB61 transcript was rapidly and dramatically induced by 6,12,24 h ethylene treatment compared with control,and was inhibited following 1-methylcyclopropene (1-MCP) treatment.These results above gave a clear data for understanding the biological function of RhMYB61, and provided a foundation for further research of molecular breeding of rose plant.

Key words: Cut roses, RhMYB61, qRT-PCR, Expression characteristics

CLC Number:

Q78
S685.03

LI Shaocui, JIANG Xinqiang, DING Aiqin, LIU Qingchao, WANG Kuiling, LI Wei, LIU Qinghua. Cloning and Expression Characteristics of RhMYB61 Gene from Rosa hybrida[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 61-68. doi: 10.7668/hbnxb.2017.05.010.

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