Exogenous BR Alleviates Drought Stress by Regulating Photosynthesis and D1 Protein in Maize Seedlings

doi:10.7668/hbnxb.2017.03.018

Abstract

Abstract: In order to reveal the protective mechanism of exogenous Brassinolide on the photosynthesis of maize seedlings under drought stress,this experiment used the Zhuyu 309 as test materials and adopted the solution culture method,studying the changes of growth parameters,chlorophyll content,photosynthetic parameters,chlorophyll fluorescence parameters and D1 protein content of maize seedlings that used 20% PEG-6000 to simulate drought stress after exogenous Brassinolide (BR) pretreatment. Compared with that of drought stress,the results of corn seedling which after the BR pretreatment showed that its height increased by 45.87%,the root length increased by 20.56%,the total dry matter accumulation increased by 8.01%,the relative water content increased by 4.50%,chlorophyll a content increased by 26.32%,and photosynthetic parameters (Pn,Gs,Ci,Tr) increased by 9.57%,38.23%,30.19% and 28.12% respectively,the activity of photosynthetic system Ⅱ (Φ_PSⅡ) increased by 20.48%,and the maximum photochemical efficiency increased by 0.66%. Photosynthetic system Ⅱ absolute electron transport rate (ETR (Ⅱ) and the relative electron transport rate of photosynthetic system Ⅱ(rETR(Ⅱ)) were increased by 20.40% and 31.02% respectively. The content of D1 protein increased by 37.34% (P <0.05). The results showed that BR treatment under drought stress condition could improve the growth and development of maize seedlings,alleviate the damage of photosynthesis system,promote the stability of D1 protein and improve the adaptability of maize seedling to drought stress.

Key words: Maize seedlings, Drought stress, Exogenous BR, Photosynthesis, D1 protein, Regulatory effect

CLC Number:

S513.01

MA Ye, YUAN Huanhuan, PEI Xinyong, RAN Wuling, FAN Xiaocong, ZHENG Boyuan, MAO Jun, YANG Qinghua, SHAO Ruixin. Exogenous BR Alleviates Drought Stress by Regulating Photosynthesis and D1 Protein in Maize Seedlings[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 118-124. doi: 10.7668/hbnxb.2017.03.018.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.03.018

http://www.hbnxb.net/EN/Y2017/V32/I3/118

References

[1] Wang Wangxia,Vinocur B,Altman A. Plant responses to drought,salinity and extreme temperatures:towards genetic engineering for stress tolerance[J]. Planta,2003,218(1):1-14.
[2] Bai L P,Sui F G,Ge T D,et al. Effect of soil drought stress on leaf water status,membrane permeability and enzymatic antioxidant system of maize[J]. Pedosphere,2006,16(3):326-332.
[3] Blum A,Ebercon A. Cell membrane stability as a measure of drought and heat tolerance in wheat[J]. Crop Science,1981,21(1):43-47.
[4] Boyer J S.Plant productivity and environment[J]. Science,1982,218(4571):443-448.
[5] 李天来,路涛,刘玉凤,等.高等植物PSⅠ和PSⅡ光抑制机理的研究进展[J]. 沈阳农业大学学报,2016, 47(5):513-519.
[6] 闫慧萍,彭云玲,赵小强,等.外源24-表油菜素内酯对逆境胁迫下玉米种子萌发和幼苗生长的影响[J]. 核农学报, 2016, 30(5):988-996.
[7] 李利红,马培芳,杨亚军,等.外源Ca^2+对高温强光胁迫下小麦叶绿体D1蛋白磷酸化及光系统Ⅱ功能的影响[J]. 应用生态学报,2010,21(3):683-688.
[8] 张会慧,张秀丽,李鑫,等.盐胁迫下桑树叶片D1蛋白周转和叶黄素循环对PSⅡ的影响[J].林业科学,2013,49(1):99-106.
[9] Bate N J,Straaus N A,Thomson J E. Expression of chloroplast photosynthesis genes during leaf senescence[J]. Physiol Plant,1990,80(2):217-225.
[10] Nic G Y,Roberlson E J,Fryer M J,et al. Response of the photosynthisis apparatus in maize leaves grown at low temperature on transfer to normal growth temperature[J]. Plant Cell Environ,1995,18(1):1-12.
[11] He J X,Wang J,Liang H G. Effects of water stress on photochemical function and protein metabolism of photosystern Ⅱ in wheat leaves[J]. Physiol Plant,1995,93(4):771-777.
[12] Hao L M,Liang H G,Wang Z L,et al. Effects of water stress and re-watering on turnover and gene expression of photosystern Ⅱ reaction center polypeptide D1 in Zea mays[J]. Functional Plant Biology,1999,26(4):375-378.
[13] Baker N R. A possible role for photosystern Ⅱ in environmental peturbations of photosynthesis[J].Phsoil Plant,1991,81(4):563-570.
[14] Lutz D,Steiger A,Godd D. Influence of aie pollutants and nutrient deficiency on D1 protein content and photosynthesis in young spruce trees[J]. Physiol Plant,1992,85(4):611-617.
[15] Bredenlamp G J,Baker N R. Temperature-sensitivity of D1 protein metabolism in isolated Zea mays chloroplasts[J]. Plant Cell and Environment,1994,17(2):205-210.
[16] Heidrun D,Herbik A,Godd D. Stressed degradation of the photosynthetic apparatus is accompanied by changes in the thylakoid protein turnover and phosphorylation[J]. Physiol Plant,1995,93(1):179-186.
[17] 尹博,王秀峰,姜春辉,等.外源油菜素内酯对番茄铜胁迫的缓解效应[J].植物营养与肥料学报,2012,18(1):162-168.
[18] 袁凌云,朱世东,赵冠艳,等.油菜素内酯诱导番茄幼苗抗冷效果的影响[J].中国农学通报,2010, 26(5):205-208.
[19] 李程,梁宝魁,王晓峰.油菜素内酯提高蔬菜作物抗逆性的研究进展[J].中国蔬菜,2015,1(11):12-18.
[20] 魏鑫,倪虹,张会慧,等.外源脱落酸和油菜素内酯对干旱胁迫下大豆幼苗抗旱性的影响[J].中国油料作物学报,2016,38(5):605-610.
[21] Mahesh K, Balaraju P, Ramakrishna B, et al. Effect of Brassinosteroids on germination and seedling growth of radish (L.) under PEG-6000 induced water stress[J].American Journal of Plant Sciences, 2013, 4(12):2305-2313.
[22] Arora P, Bhardwaj R, Kanwar M K. Effect of 24-epibrassinolide on growth, protein content and antioxidative defense system of Brassica juncea L. subjected to cobalt ion toxicity[J].Acta Physiologiae Plantarum, 2012, 34(5):2007-2017.
[23] 吴雪霞,查丁石,朱宗文,等.外源24-表油菜素内酯对盐胁迫下茄子种子萌发和幼苗生理特性的影响[J].植物生理学报,2011,47(6):607-612.
[24] Guo J W,Wei H M,Wu S F. Effects of low temperature on the distribution of excitation energy in photosystem and the phosphorylation of thylakoid membrane proteins in rice[J]. Acta Biophysica Sinica,2006,22(3):197-202.
[25] Du L F,Sun X,Pan Y H,et al. Effect of Calcium ion on O₂-evolution activity of PSⅡ[J]. China Science,1995,38(12):1439-1447.
[26] Nielsen D C,Vigil M F,Benjamin J G. The variable response of dryland corn yield to soil water content at planting[J]. Agricultural Water Management,2009,96(2):330-336.
[27] Mirzaee M, Moieni A, Ghanati F. Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola (Brassica napus L.) cultivars[J].Journal of Agricultural Science & Technology, 2013, 15(3):593-602.
[28] 李强,张林森,刘辉,等.水分胁迫对玉米苗期形态及生理指标的影响[J].甘肃农业科技,2014(1):36-38.
[29] Kai R L, Feng C H. Effects of brassinolide on drought resistance of Xanthoceras sorbifolia, seedlings under water stress[J]. Acta Physiologiae Plantarum, 2011, 33(4):1293-1300.
[30] M. Koćová, O Rothová, D Holá, et al. The effects of brassinosteroids on photosynthetic parameters in leaves of two field-grown maize inbred lines and their F1 hybrid[J].Biologia Plantarum, 2010, 54(4):785-788.
[31] 吴晓丽,罗立津,黄丽,等.水杨酸和油菜素内酷对花椰菜幼苗生长及抗旱性的影响[J].PEG-6000地区农业研究,2011,29(2):168-172.
[32] Vardhini B V,Rao S S R. Amelioration of osmotic stress by brassinosteroids on seed germination and seedling growth of three varieties of sorghum[J].Plant Growth Regulation,2003,41(1):25-31.
[33] Sairam R K. Effects of homobrassinolide application on plant metabolism and grain yield under irrigated and moisture-stress conditions of two wheat varieties[J]. Plant Growth Regulation,1994,14(2):173-181.
[34] 郑静静,赵会杰,胡巍巍,等.高温强光对小麦叶绿体Deg1蛋白酶和D1蛋白的影响及水杨酸的调节作用[J].生态学报,2013,33(9):2930-2935.
[35] Zhao H J,Zhao X J,Ma P F,et al. Effects of salicylic acid on protein kinase activity and chloroplast D1 protein degradation in wheat leaves subjected to heat and high light stress[J]. Acta Ecologica Sinica,2011,31(5):259-263.
[36] Mizusawa N,Tomo T,Satoh K,et al. Degradation of the D1 protein of photosystem Ⅱ under illumination in vivo:two different pathways involving cleavage or intermolecular cross-linking[J]. Biochemistry,2003,42(33):10034-10044.
[37] 郑静静,杨丽,苏小雨,等.水杨酸对高温强光下小麦叶绿体蛋白酶Deg5和PSⅡ功能的调节作用[J].生态学报,2014,34(24):7350-7355.
[38] Ghotbi-Ravandi A A,M Shahbazi,M Shariati,et al. Effects of mild and severe drought stress on photosynthetic efficiency in tolerant and susceptible barley(Hordeum vulgare L.)genotypes[J]. Journal of Agronomy and Crop Science,2014,200(6):403-415.

Please choose a citation manager

Content to export