Abstract: A next-generation,Illumina-based sequencing approach was used to characterize the bacterial community development of the long-term straw returning soil.Five treatments (soil without fertilizer,wheat and corn straw returning,wheat straw returning,manure,and wheat and corn straw returning) were tested in this study.In this study,the microbial diversity of soil fungi was studied by ITS rRNA PCR technology.The results showed that the community was composed of 3 dominant groups (Ascomycota,Zygomycota,Basidiomycota).Principal component analyses revealed that the microbiota were significantly different among treatments.The WCN treatment could significantly increase soil total nitrogen,available phosphorus,available potassium,and organic carbon content.The WCN treatment showed relatively higher soil total N,available P,available K,and organic carbon and invertase,urease,and cellulase activities than WC treatment,and this treatment also had the higher maize yield.Our experimental results suggested that application of N fertilizer at straw returning soil had significantly higher soil fertility,enzyme activities than straw returning,alone,which resulted in a different bacterial community composition.

Key words: Long-term experiment, ITS rRNA, Soil fungal community, Straw returning

CLC Number: 

• S158