Responses of Growth and Physiological Features,Photosynthetic Function in Overexpression and Inhibition of Expression of Transgenic Tobacco Plants with 2-Cys Prx to the Simulated Acid Rain

doi:10.7668/hbnxb.2016.02.010

Abstract

Abstract: The overexpression and inhibition of expression of transgenic tobacco plant with 2-Cys Prx (Longjiang 911), non transgenic tobacco were chosen to investigate the effects of simulated acid rain on their growth, physiological features and photosynthesis.The results showed that simulated acid rain at pH 4.0 had no significant effects on the plant height, leaf area and specific leaf weight, but at pH 2.5, the plant height of overexpression and inhibition of expression of transgenic tobacco plant with 2-Cys Prx reduced significantly, With the pH value of the simulated acid rain decreased, chlorophyll content, nitrate reductase activity, soluble protein content, superoxide dismutase and ascorbate peroxidase activity, net photosynthetic rate(Pn), stomatal conductance(Gs), transpiration rate(Tr), the actual photochemical efficiency of PSⅡ(Φ_PSⅡ), photochemical quenching coefficient(qP), potential photochemical efficiency of PSⅡ(Fv/Fo) and cell membrane stability index decreased, the non-photochemical quenching(qN) and hydrogen peroxide content increased, but at the same pH value, these above-mentioned indexes of overexpression of transgenic tobacco plant with 2-Cys Prx were better than inhibition of expression of transgenic tobacco plant with 2-Cys Prx and non transgenic tobacco plant.The results suggest that enhanced expression of 2-Cys Prx in tobacco plant can promote the capability of tobacco against acid rain, the capability of utilizing nitrate nitrogen, and remove of excess H₂O₂ while APX activity decreases, and enhance the photosynthetic capability of tobacco.

Key words: Tobacco, 2-Cys Prx gene, Simulated acid rain, Photosynthetic function

CLC Number:

S572.01

DAI Xin, ZHANG Xiuli, HU Juwei, SUN Guangyu. Responses of Growth and Physiological Features,Photosynthetic Function in Overexpression and Inhibition of Expression of Transgenic Tobacco Plants with 2-Cys Prx to the Simulated Acid Rain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 52-59. doi: 10.7668/hbnxb.2016.02.010.

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