Preliminary Analysis on Sequence and Function of Rice Gene Encoding SAND-domain Protein

doi:10.7668/hbnxb.2016.02.001

Abstract

Abstract: To study the sequence and function of rice SAND domain protein, the gene encoding SAND was identified by Blast against the rice genome, and then over-expression vector was constructed to study its gene function by genetic transformation.The results found that the rice genome contains a gene LOC_Os01g57240 (named as OsULT1), which encoded amino acid sequence containing SAND domain and ULT B-box consensus sequence.The amino acid sequence similarity between OsULT1 and ULTs of other plants varied from 49.3% to 92.3%, they were highly conservative. The 35S::OsULT1 transgenic lines generated some mutant spikilets, which included under-developed or degenerated paleas, over-developed locidules, the increased number of locidules, the variant number of stamen, two pistils, or extra floret.The results indicates OsULT1 should play important roles in regulation of floral meristem identity in rice.

Key words: Rice(Oryza sativa L.), Floral development, SAND-domain, Gene, Sequence

CLC Number:

S511.03
Q78

CHEN Zhixiong, DAI Shuangfeng, XIA Changxuan, XIE Haimei, LI Yajuan. Preliminary Analysis on Sequence and Function of Rice Gene Encoding SAND-domain Protein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 1-6. doi: 10.7668/hbnxb.2016.02.001.

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