Temporal and Special Expression of Genes Related to Anther Development of Fax Flower Buds

doi:10.7668/hbnxb.2015.05.017

Abstract

Abstract: In this paper,the real time quantitative PCR technology and in situ hybridization method were adopted,temporal and special expression of three candidate genes G-E07-830, G-E07-330 and G-E07-100 in fertile and sterile flower buds separated from sterile flax of dominant genomic male sterile flax.The results showed that the three genes were specifically expressed in the tapetum of fertile and sterile flower buds.They were expressed at significantly higher levels in the early stage sterile buds than other developmental stages.The results provides some valuable information for the revealing of the molecular mechanism of pollen development and male sterility in flax.

Key words: Flax, Flower bud, Gene temporal and special expression, Real time quantitative PCR, In situ hybridization

CLC Number:

S563.2

JIANG Cai, YI Liu-xi, GAO Feng-yun, JIA Xiao-yun, ZHANG Hui, ZHANG Li-hua, REN Long-mei, ZHOU Yu, ZHAO Xiao-qing, Siqin Bateer. Temporal and Special Expression of Genes Related to Anther Development of Fax Flower Buds[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 104-107. doi: 10.7668/hbnxb.2015.05.017.

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