DNA Fingerprints Construction and Genetic Diversity Analysis of Short-season Cotton by SSR Markers

doi:10.7668/hbnxb.2014.04.016

Abstract

Abstract: In Yangtze river basin,little SSR primers and little genetic information is available for DNA fingerprints and diversity of short season cotton germplasms introduced from the northern of China,so,in present research,fifteen short season cotton introduced from Institute of Cotton Research,Chinese Academy of Agricultural Sciences(ICR,CAAS)were used as materials in construction of DNA fingerprinting and analysis of genetic diversity with simple sequence repeat(SSR)makers.Sixty-eight primer pairs with good repeatability,high polymorphism,almost even distribution on the 26 chromosomes were screened out from 214 pairs of candidate SSR primers to genotype the 15 materials,178 effective allelic loci were detected with an average of 2.62;Primers polymorphism information content(PIC)value ranged from 0.244 9 to 0.906 1 with an average of 0.693 8.Variety fingerprint analysis results showed that 12 cultivars could be differentiated by specific or characteristic primer,the rest 3 cultivars could be identified by primer combinations.In the end,four pairs of primers combinations to be detected in 21 polymorphic bands with an average of 5.25 could completely differentiate the 15 short season cotton cultivars from each other.NTSYS-pc2.1 software was used to analyze genetic diversity of the 15 cultivars,the results indicated that genetic similarity coefficient of the 15 cultivars ranged from 0.45 to 0.79,with an average of 0.62,the 15 materials can be divided into five major categories at the similarity coefficient of 0.68.combined with the pedigree of clustering to be carried on the system analysis,which provided basis for the short season cotton identification and parental material selection.

Key words: Short-season cotton, SSR, Fingerprint, Genetic diversity

CLC Number:

S562.03

CHEN Hao-dong, XIAO Cai-sheng, LI Xiang, KUANG Zheng-cheng, WANG Wei, LI Yu-qiang. DNA Fingerprints Construction and Genetic Diversity Analysis of Short-season Cotton by SSR Markers[J]. journal1, 2014, 29(4): 98-104. doi: 10.7668/hbnxb.2014.04.016.

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