Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl(t) in Rice

doi:10.7668/hbnxb.2014.04.007

Abstract

Abstract: Leaf morphology is one of key components for rice plant architecture,so excavation,mapping and cloning related mutant genes are very important for revealing mechanism of leaf development and breeding new rice varieties with ideotype and super-high yield.In this study,the narrow and rolled mutant ntl_(t) was found from the improved line of rice stripe disease for Wuyunjing No.7 and the mutant phenotype genetic analysis and gene mapping was researched.Compared with wild type,the mutant showed multipe abnormal phenotypes,such as shorter leaf length and width,rolled leaf,dwarfism,more tillers,reduced panicle length,branch number,filled grain number per spike,spikelets per spike,seed-setting rate and 1000-grain weight.Genetic analysis indicated that the mutant was controlled by a single recessive gene.Using 500 recessive mutant individuals in the F₂ population derived from the cross between Fuhui 838 and nrl_(t),the gene NRL _(t) was located to a confined region of 160 kb flanked by two microsatellite markers RM11-01 and RM11-11 on the short arm of chromosome 11.Based on the annotation result of rice genome,26 open reading frames(ORFs)were predicted in this region.Sequencing analysis showed that the homeobox3A gene reported might be the potential candidate gene.These results are very valuable for further study on this gene.

Key words: Oryza sativa L., Narrow and rolled leaf, Mutant, Genetic analysis, Gene mapping

CLC Number:

CHEN Tao, LIU Yan-qing, ZHANG Ya-dong, ZHU Zhen, ZHAO Qing-yong, ZHOU Li-hui, YAO Shu, YU Xin, ZHAO Ling, WANG Cai-lin. Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl_(t) in Rice[J]. journal1, 2014, 29(4): 37-43. doi: 10.7668/hbnxb.2014.04.007.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.04.007

http://www.hbnxb.net/EN/Y2014/V29/I4/37

References

[1] Hudson A.Axioms and axes in leaf formation [J].Current Opinion in Plant Biology,1999,2(1):56-60.
[2] 袁隆平.杂交水稻超高产育种[J].科学中国人,1998,12(6):14-16.
[3] 陈代波,程式华,曹立勇.水稻窄叶性状的研究进展[J].中国稻米,2010,16(3):1-4.
[4] 余东,吴海滨,杨文韬,等.水稻单侧卷叶突变体B157遗传分析及基因初步定位[J].分子植物育种,2008,6(2):220-226.
[5] 曾生元,郭旻,李敏,等.一个水稻动态窄叶突变体的鉴定和基因定位[J].科学通报,2010,55(21):2106-2111.
[6] 潘存红,李磊,陈宗祥,等.一个水稻卷叶基因 rl_(t)的精细定位[J].中国水稻科学,2011,25(5):455-460.
[7] Zou L P,Sun X H,Zhang Z G,et al.Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice [J].Plant Physiology,2011,156(3):1589-1602.
[8] Wu R H,Li S B,He S,et al.CFL1,a WW domain protein,regulates cuticle development by modulating the function of HDG1,a class IV homeodomain transcription factor,in rice and arabidopsis[J].Plant Cell,2011,23(9):3392-3411.
[9] 王德仲,桑贤春,游小庆,等.水稻细卷叶突变体nrl2_(t)的遗传分析和基因定位[J].作物学报,2011,37(7):1159-1166.
[10] Shi Z Y,Wang J,Wan X,et al.Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit [J].Planta,2007,226(1):99-108.
[11] Fujino K,Matsuda Y,Ozawa K,et al. NARROW LEAF 7 controls leaf shape mediated by auxin in rice [J].Molecular Genetics Genomics:MGG,2008,279(5):499-507.
[12] Qi J,Qian Q,Bu Q Y,et al.Mutation of the rice Narrow leaf1 gene,which encodes a novel protein,affects vein patterning and polar auxin transport [J].Plant Physiology,2008,147(4):1947-1959.
[13] 李娟,李东宣,甘树仙,等.一个新的水稻窄叶突变体的遗传分析和基因定位[J].分子植物育种,2011,9(2):143-149.
[14] Chen M L,Luo J,Shao G N,et al.Fine mapping of a major QTL for flag leaf width in rice, qFLW4,which might be caused by alternative splicing of NAL1 [J].Plant Cell Reports,2012,31(5):863-872.
[15] Fujita D,Trijatmiko K R,Grubanzo A T,et al. NAL1 allele from a rice landrace greatly increases yield in modern indica cultivars [J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(51):20431-20436.
[16] Li L,Shi Z Y,Li L,et al.Overexpression of ACL1 (abaxially curled leaf 1)increased bulliform cells and induced abaxial curling of leaf blades in rice [J].Molecular Plant,2010,3(5):807-817.
[17] 李仕贵,马玉清,何平,等.一个未知的卷叶基因的识别和定位[J].四川农业大学学报,1998,16(4):391-393.
[18] 邵元健,陈宗祥,张亚芳,等.一个水稻卷叶主效QTL的定位及其物理图谱的构建[J].遗传学报,2005,32(5):501-506.
[19] Shi Y F,Chen J,Liu W Q,et al.Genetic analysis and gene mapping of a new rolled-leaf mutant in rice(Oryza sativa L.)[J].Science in China Series C:Life Sciences,2009,52(9):885-890.
[20] Xiang J J,Zhang G H,Qian Q,et al. SEMI-ROLLED LEAF1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells [J].Plant Physiology,2012,159(4):1488-1500.
[21] Zhang G H,Xu Q,Zhu X D,et al. SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development [J].Plant Cell,2009,21(3):719-735.
[22] Yan C J,Yan S,Zhang Z Q,et al.Genetic analysis and gene fine mapping for a rice novel mutant(rl9_(t))with rolling leaf character [J].Chinese Science Bulletin,2006,51(1):63-69.
[23] Yan S,Yan C J,Zeng X H,et al. ROLLED LEAF 9,encoding a GARP protein,regulates the leaf abaxial cell fate in rice [J].Plant Molecular Biology,2008,68(3):239-250.
[24] Luo Z K,Yang Z,Zhong B,et al.Genetic analysis and fine mapping of a dynamic rolled leaf gene, RL10_(t),in rice(Oryza sativa L.)[J].Genome,2007,50(9):811-817.
[25] 罗远章,赵芳明,桑贤春,等.水稻新型卷叶突变体rl12_(t)的遗传分析和基因定位[J].作物学报,2009,35(11):1967-1972.
[26] Fang L K,Zhao F,Cong Y,et al. Rolling-leaf14 is a 2OG-Fe(Ⅱ)oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves[J].Plant Biotechnology Journal,2012,10(5):524-532.
[27] 张帆涛,方军,孙昌辉,等.水稻矮秆突变体dtl1的分离鉴定及其突变基因的精细定位[J].遗传,2012,34(1):79-86.
[28] Cho S H,Yoo S C,Zhang H T,et al.The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A)and function in leaf,spikele,tiller and lateral root development [J].New Phytologist,2013,198(4):1071-1084.
[29] 李楠,桑贤春,赵芳明,等.水稻窄叶突变体nal_(t)的表型分析与基因定位[J].植物学报,2010,45(2):157-161.
[30] Wang D K,Liu H Q,Li K L,et al.Genetic analysis and gene mapping of a narrow leaf mutant in rice(Oryza sativa L.)[J].Chinese Science Bulletin,2009,54(5):752-758.
[31] Li M,Xiong G Y,Li R,et al.Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth [J].Plant Journal,2009,60(6):1055-1069.
[32] Hu J,Zhu L,Zeng D L,et al.Identification and characterization of NARROW and ROLLED LEAF1,a novel gene regulating leaf morphology and plant architecture in rice [J].Plant Molecular Biology,2010,73(3):283-292.
[33] 朱德峰,林贤青,曹卫星,不同叶片卷曲度杂交水稻的光合特性比较[J].作物学报,2001,27(3):329-333.
[34] Dellaporta S L,Wood J,Hicks J B.A plant DNA minipreparation.VersionⅡ[J].Plant Molecular Biology Reporter,1983,1(1):19-21.
[35] Chen X,Temnykh S,Xu Y,et al.Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)[J].Theoretical and Applied Genetics,1997,95(4):553-567.
[36] 李强,万建民.SSRHunter,一个本地化的SSR位点搜索软件的开发[J].遗传,2005,27(5):808-810.
[37] Lander E S,Green P,Abrahamson J,et al.MAPMAKER:an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations [J].Genomics,1987,1(2):174-181.
[38] 刘仁虎,孟金陵.MapDraw,在Excel中绘制遗传连锁图的宏[J].遗传,2003,25(3):317-321.
[39] 严松,严长杰,顾铭洪.植物叶发育的分子机理[J].遗传,2008,30(9):1127-1135.
[40] Zhang B S,Tian F,Tan L B,et al.Characterization of a novel high-tillering dwarf 3 mutant in rice [J].Journal of Genetics and Genome,2011,38(9):411-418.

Please choose a citation manager

Content to export