Cloning and Expression Analysis of TaMSR Gene from Triticum aestivum

doi:10.7668/hbnxb.2014.04.006

Abstract

Abstract: In search for fertility gene in wheat,three cDNAs that homologous to AtMS2 and putative OsMS2,designated as TaMSR-1, TaMSR-2 and TaMSR-3 were cloned from wheat anther by homologogy-based cloning approach. TaMSR-1, TaMSR-2 and TaMSR-3 contain open reading frames of 1 842,1 824,1 830 bp encoding proteins of 613,607,609 amino acid residues including two male sterile conserved domains:NAD-binding domain and male sterile C-terminal domain,respectively.The deduced amino acid sequence of TaMSR had high similarities with OsMSR2 and AtMSR2.Using Chinese_Spring_Deletion line, TaMSR-1, TaMSR-2 and TaMSR-3 genes were located on chromosome 4AS,4DL and 4BL,respectively.Semi-quantitative RT-PCR analysis revealed that TaMSR gene was anther specific expressed gene.These results suggested that TaMSR may plays an important role in wheat anther development.

Key words: Wheat, TaMSR gene, Expression analysis, Chromosomal localization

CLC Number:

YU Yue-hua, ZHANG Yue-qiang, HAIReguli Abulizi, LIU Zhen-fang, LIANG Xiao-li. Cloning and Expression Analysis of TaMSR Gene from Triticum aestivum[J]. journal1, 2014, 29(4): 32-36. doi: 10.7668/hbnxb.2014.04.006.

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References

[1] 李惟基.遗传学[M].北京:中央广播电视大学出版社,2010:131-135.
[2] Aarts M G,Hodge R,Kalantidis K,et al.The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes[J].The Plant Journa:for Cell and Molecular Biology,1997,12(3):615-623.
[3] Aarts M G,Dirkse W G,Stiekema W J,et al.Transposon tagging of a male sterility gene in Arabidopsis [J].Nature,1993,363(6431):715-717.
[4] Hodge R,Paul W,Draper J,et al.Cold-plaque screening:a simple technique for the isolation of low abundance,differentially expressed transcripts from conventional cDNA libraries[J].The Plant Journal,1992,2(2):257-260.
[5] 李德谋,侯磊,罗小英,等.甘蓝型油菜隐性核不育两用系S45AB中与 MS2Bnap 基因同源片段的克隆及序列分析[J].作物学报,2002,28(1):1-5.
[6] 斯钦巴特尔,张辉,哈斯阿古拉,等.亚麻中雄性不育基因同源序列 MS2-F 的克隆和表达分析[J].植物生理学通讯,2008,44(5):897-902.
[7] 方子君,石其龙,杨仲南,等.水稻 OsMS2 基因在花药发育中的功能分析[J].植物学通报,2008,25(6):665-672.
[8] 王文锋,王红霞,穆灵敏.花粉育性基因 MS2 的克隆与RNAi载体构建[J].安徽农业科学,2009,37(20):9396-9397.
[9] 赵昌平.中国杂交小麦研究现状与趋势[J].中国农业科技导报,2010,12(2):5-8.
[10] 于月华,王莉萍,高文伟,等.小麦盐胁迫相关基因的克隆与表达分析[J].西北植物学报,2012,32(6):1073-1078.
[11] Schiefthaler U,Balasubramanian S,Sieber P, et al.Molecular analysis of NOZZLE,a gene involved in pattern formation and early sporogenesis during sex organ development in Arabidopsis thaliana [J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(20):11664-11669.
[12] Yang W C,Ye D,Xu J,et al.The Sporogenesis gene of Arabidopsis is required for initiation of sporogenesis and encodes a novel nuclear protein[J].Genes& Development,1999,13(16):2108-2117.
[13] Zhang W,Sun Y L,Timofejeva L, et al.Regulation of Arabidopsis tapetum development and function by DYSFUNCTIONAL TAPETUM(DYT1) encoding a putative bHLH transcription factor[J].Development(Cambridge England),2006,133(16):3085-3095.
[14] Wilson Z A,Morroll S M,Dawson J, et al.The Arabidopsis MALE STERILITY1 (MS1) gene is a transcriptional regulator of male gametogenesis,with homology to the PHD-finger family of transcription factors[J].The Plant Journal:for Cell and Molecular Biology,2001,28(1):27-39.
[15] Yang C Y,Vizcay-Barrena G,Conner K,et al.MALE STERILITY1 is required for tapetal development and pollen wall biosynthesis[J].The Plant Cell,2007,19(11):3530-3548.
[16] Ito T,Nagata N,Yoshiba Y,et al. Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development[J].The Plant Cell,2007,19(11):3549-3562.
[17] Zhao D Z,Wang G F,Speal B, et al.The excess microsporocytes1 gene encodes a putative leucine-rich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther [J].Genes& Development,2002,16(15):2021-2031.
[18] Wijeratne A J,Zhang W,Sun Y J,et al.Differential gene expression in Arabidopsis wild-type and mutant anthers:insights into anther cell differentiation and regulatory networks[J].The Plant Journal:for Cell and Molecular Biology,2007,52(1):14-29.
[19] Qi L L,Echalier B,Chao S, et al. A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat[J].Genetics,2004,168(2):701-712.

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