Abstract: In order to study factors that influence the separation and culture of tilapia peritoneal macrophages,and to recognition the characteristic of tilapia peritoneal macrophages.In the study,We separated macrophages from peritoneal cavity of Tialpia(Oreochromis niloticus♀×Oreochromis aureus♂)after intraperitoneal injected with squalene in the condition of difference growth temperature;The cell characteristic was observed by screening cell medium and Wright's staining.The results show the macrophages were easily obtained in the condition of 19~25℃,Separation result was obviously affected when water temperature was aboved 28℃;It was a optimization period for separation between 48-72 h after injected squalene;Serum of tilapia play an important role in the tilapia peritoneal macrophages invitro cultrue and fetal bovine serum was not fit;The characteristic of tilapia peritoneal macrophage was similar to mammalian macrophage,irregularly shaped with a low nucleus to cytoplasm ratio,adherent to plastic and glass,able to form multi-nucleated giant.The work laid a foundation for the further research of immunological function about tilapia peritoneal macrophage.

Key words: Tilapia, Peritoneal macrophages, Separation, Cultivation

CLC Number: 

• S965.125