Preparation and Application of Peptide Antibody against Porcine Circovirus-like Agent P1

doi:10.7668/hbnxb.2011.05.017

Abstract

Abstract: To generate and investigate rabbit polyclonal antibody against porcine circovirus-like agent P1.The certain epitope peptides were chosen and synthesized,and then coupled to keyhole limpet hemocyanin(KLH)to increase antigenicity.New Zealand White Rabbits were immunized with KLH conjugated peptides.The polyclonal antibodies elicited were charactered by ELISA and tested by immunohistochemical techniques in etiological diagnosis of P1.The polyclonal antibodies against porcine circovirus-like agent P1 were generated successfully.Immunohistochemistry showed the antibodies could specifically react with P1 proteins.The synthesized aim peptides can be used to immunized rabbit in order to generate polyclonal antibodies against porcine circovirus-like agent P1.The antibodies prepared can be used as potential tools to study etiological diagnosis of P1.

Key words: P1, Synthesized peptides, Polyclonal antibody

CLC Number:

S852.65

WEN Li-bin, HE Kong-wang, YANG Han-chun, LIU Meng-ya. Preparation and Application of Peptide Antibody against Porcine Circovirus-like Agent P1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 83-86. doi: 10.7668/hbnxb.2011.05.017.

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