EST-SSR Information Analysis and Markers Development in Watermelon Based on Public Databases

doi:10.7668/hbnxb.2011.02.019

Abstract

Abstract: In order to explore a new co.dominant molecular markers to study the genetic basic research in watermel· on.We analysed a total of 8 619 watermelon ESrrs from GenBank and sought out 202 SSR contained in 191 EST se· quences.The frequency of the SSRs was 2.31%and mean the distribution density was 1/19.1 kb in watermelon.The dominant repeat motifs were dinucleotide and trinucleotide.AG/TG was the most repeat type in dinucleotide(46%)and AAG was the most repeat type in trinucleotide(43%).卟e most motif repeat times were 4(17.8%),7(15.3%)and 11 (14.9%).The length was mainly distributed between 20 to 30 bp.We obtained 24 primer pairs from 202 EST-SSRs in watermelon.Then utilized albino lethal near.isogenic lines of watermelon for verify these primers.We obtained 16 effecrive amplified primer pairs.accouting for 66.7%of the total number of designed primer pairs.The results suggest that use of public EST databases to develop EST.SSR primers of watermelon areeffective method.

Key words: Watermelon, EST, EST-SSR, Primer

CLC Number:

BAO Wen-feng, WANG Ji-ming, SHANG Jian-li, MA Shuang-wu. EST-SSR Information Analysis and Markers Development in Watermelon Based on Public Databases[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 85-89. doi: 10.7668/hbnxb.2011.02.019.

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